GPC4 Knouckout HAP1 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout population targeting the GPC4 gene in the HAP1 human cell line. This mixed population of edited cells provides a loss-of-function model for investigating glypican-4 biology without requiring single-cell cloning, enabling pool-based functional studies.
HAP1 cells are a near-haploid human cell line derived from the male chronic myeloid leukemia KBM-7 line. Their near-haploid karyotype reduces genetic redundancy, making them particularly suitable for genetic screens and functional genomics applications. These suspension-adapted cells retain key signaling pathways and offer a simplified genetic background for studying signaling networks.
Glypican-4 (GPC4) is a heparan sulfate proteoglycan attached to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. It acts as a co-receptor or reservoir for multiple growth factors, modulating Wnt, Hedgehog, FGF, and BMP signaling. GPC4 interacts with ligands such as Wnt3a, Sonic Hedgehog (SHH), FGF2, and BMP4, and facilitates their presentation to cognate receptors including Frizzled/LRP5/6, Patched/Smoothened, FGFR, and BMP receptors. Downstream, GPC4 influences transcriptional targets such as MYC and CCND1 (via Wnt/??-catenin), GLI1 and PTCH1 (via Hedgehog), and FOS/EGR1 (via MAPK/ERK). Consequently, GPC4 knockout is predicted to impair ligand-dependent receptor activation and attenuate these signaling cascades.
Disruption of GPC4 in the HAP1 near-haploid background creates a powerful platform for dissecting glypican-4-dependent signaling mechanisms. The simplified genome allows clear delineation of pathway contributions, while the leukemic origin provides a context for studying proliferative and oncogenic signaling. This knockout population is expected to exhibit reduced proliferative capacity, altered differentiation markers, and diminished responsiveness to Wnt and Hedgehog stimuli, enabling precise interrogation of co-receptor function in a human cell system.
Researchers can employ GPC4 Knochout HAP1 Polyclonal Cells in a range of applications, including Wnt and Hedgehog pathway reporter assays (TOP/FOP flash, Hedgehog reporters), Western blot analysis of ??-catenin, GLI1, or phospho-ERK, and RT-qPCR quantification of target genes like MYC and CCND1. Transcriptomic analysis via RNA-seq reveals global expression changes, while flow cytometry enables assessment of proliferation and apoptosis. Functional assays such as migration, invasion, and drug sensitivity testing further elucidate the role of GPC4 in cancer biology and developmental processes. For additional information or technical assistance regarding this product, please contact Ascent Research.
