The GPR107 Knockout HeLa Polyclonal Cells product consists of a CRISPR/Cas9-edited polyclonal population of HeLa cells carrying targeted disruption of the GPR107 gene. This loss-of-function model enables the study of orphan G protein-coupled receptor 107 function within a defined host background. The polyclonal knockout format provides a heterogeneous gene-edited population suitable for pooled functional experiments and pathway analysis, without clonal selection artifacts.
HeLa cells, a human epithelial cell line originally isolated from a cervical adenocarcinoma, serve as a widely used model for human cell biology, cancer research, and signal transduction studies. These adherent cells exhibit rapid proliferation and a well-characterized genome, facilitating reliable interpretation of genetic perturbations. The HeLa background endogenously expresses core Hedgehog pathway components and supports high transfection efficiency, making it a robust platform for GPR107 knockout experiments.
GPR107 encodes an orphan G protein-coupled receptor that is indispensable for Hedgehog signaling. Mechanistically, it interacts with Smoothened (SMO) and recruits the catalytic subunit of PKA (PRKACA) to regulate SMO trafficking and GLI activation. Following SHH ligand binding, GPR107 relieves SUFU-dependent repression of GLI transcription factors, promoting expression of downstream effectors such as GLI1, PTCH1, and HHIP. Additionally, GPR107 associates with GNAS, integrating Hedgehog signals with cAMP/PKA cascades.
Disruption of GPR107 in HeLa cells abrogates Hedgehog pathway responsiveness, creating a powerful model to dissect GPCR-mediated signal transduction in an epithelial cancer context. Given the role of aberrant Hedgehog signaling in tumorigenesis and neural tube defects, this knockout system enables investigation of pathway dependencies, proliferation control, and oncogenic mechanisms. The HeLa cervical cancer origin provides a relevant background to assess the interplay between GPR107 loss and tumor cell behavior.
This polyclonal knockout population is suitable for qPCR analysis of Hedgehog target genes, Western blotting of SMO and GLI, luciferase reporter assays, and SMO localization studies by immunofluorescence. Co-immunoprecipitation can confirm GPR107-PKA complexes, and cell proliferation assays can evaluate Hedgehog-dependent growth. The model is ideal for pharmacological inhibitor screening and genetic interaction studies. For additional information, contact Ascent Research.
