The GPR75 Knockout Ca Ski Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell pool targeting the human GPR75 gene in the Ca Ski cervical carcinoma cell line. This product provides a heterogeneous population of cells with gene disruption introduced via non-homologous end joining, enabling loss-of-function analysis without clonal selection. It is designed for researchers investigating the biological roles of GPR75, an orphan G protein-coupled receptor linked to metabolic regulation and body weight homeostasis.
The host Ca Ski cell line was established from a metastatic cervical epidermoid carcinoma and harbors integrated human papillomavirus type 16 (HPV16) genomes. As an adherent epithelial model, Ca Ski cells are widely employed in studies of cervical cancer pathogenesis, HPV oncogene function, and tumor cell biology. Their well-characterized growth properties and HPV status make them a robust platform for examining host-virus interactions and oncogenic signaling networks.
GPR75 is an orphan GPCR predicted to signal through G??s proteins, leading to adenylyl cyclase activation and increased intracellular cAMP levels. Downstream events may involve cAMP-dependent signaling cascades and recruitment of ??-arrestin. While its endogenous ligand remains unknown, the receptor’s association with metabolic pathways positions it as a potential regulator of energy balance. Representative pathway components include GPR75, G??s, adenylyl cyclase, and cAMP, forming a canonical GPCR?CcAMP axis that influences cellular metabolism.
Introduction of GPR75 knockout in the Ca Ski background allows interrogation of this receptor’s role within an HPV-positive epithelial tumor context. Because metabolic reprogramming is a hallmark of cancer, this model can be used to explore whether GPR75-mediated signaling contributes to the altered bioenergetics of cervical carcinoma cells. Additionally, the combination of HPV oncoprotein expression and disrupted GPCR function offers a unique system for dissecting crosstalk between viral oncogenesis and host metabolic pathways.
Researchers can employ this knockout model in a range of functional assays, including RT-qPCR and Western blotting for confirming gene expression changes, immunofluorescence for localization studies, and cAMP assays to assess downstream signaling. Metabolic flux analysis and proliferation assays further enable evaluation of receptor impacts on cellular energetics and growth. This product is well suited for mechanistic studies, drug target validation in obesity research, and exploration of GPR75 function in cancer metabolism. For additional information or technical support, please contact Ascent Research.
