The GPRIN1 Knockout Jurkat Polyclonal Cells are a human polyclonal knockout cell population derived from the Jurkat T lymphoblastoid line via CRISPR/Cas9-mediated disruption of the GPRIN1 gene. This loss-of-function model comprises a heterogeneous mix of edited alleles, circumventing clonal artifacts and enabling robust investigation of GPRIN1-dependent pathways.
The parental Jurkat cell line was established from the peripheral blood of a 14-year-old male with acute T cell leukemia and serves as a principal model for T cell signaling, proliferation, and apoptosis. Its well-defined signaling cascades and adaptability to high-throughput formats make it ideal for studying GPCR-mediated cytoskeletal regulation. Introducing GPRIN1 knockout in Jurkat cells provides a platform to assess how this neuronal regulator impacts immune cell function.
GPRIN1 functions as a key downstream mediator of GPCR signaling, directly interacting with activated G?? subunits (G??o, G??i) and transducing signals to the actin and microtubule cytoskeletons. It is regulated by GPCR ligands and NGF/TrkA signaling and, through activation of RhoGTPases such as Rac1 and Cdc42, modulates actin polymerization, focal adhesion dynamics, and cell morphology. GPRIN1 also associates with G?¦? and tubulin, positioning it at a critical node for coordinating cytoskeletal rearrangements essential for neurite outgrowth and, potentially, immune cell adhesion and migration.
In the Jurkat T-cell context, GPRIN1 knockout enables the study of GPCR-dependent cytoskeletal control in immune cells. Although GPRIN1 is predominantly characterized in neurons, its expression and interaction partners??including actin, tubulin, and RhoGTPases??hint at conserved roles in T cell morphology and motility. This model can reveal how GPRIN1 contributes to immune synapse formation, chemotaxis, and adhesion, bridging neurobiology and immunology.
This knockout cell product supports diverse applications: mapping GPCR-to-cytoskeleton signaling cascades, assessing T cell adhesion and Transwell migration, and conducting drug screens for neuropsychiatric disorders targeting GPRIN1. Compatible assays include Western blot, RT-qPCR, F-actin immunofluorescence, GTPase activity assays, co-immunoprecipitation, and flow cytometry for activation markers. These polyclonal knockout cells offer a versatile system for both fundamental research and therapeutic development. For inquiries and ordering, please contact Ascent Research.
