GPSM1 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HAP1 human cell line. The product features targeted disruption of the GPSM1 gene, creating a loss-of-function model for investigating its cellular roles. As a polyclonal pool, these cells are ideal for bulk biochemical and phenotypic assays, avoiding artifacts associated with single-cell clonal isolation.
The HAP1 host cell line is a near-haploid, suspension-adapted model originating from a patient with chronic myeloid leukemia (CML). Its haploid karyotype facilitates recessive genetic screens and simplifies genotype?Cphenotype correlations, making it a powerful platform for functional genomics. HAP1 cells are well-characterised and widely used for CRISPR-based screens, drug target validation, and studies of cell signaling in a leukemic background.
GPSM1 (also known as AGS3) functions primarily as a guanine nucleotide dissociation inhibitor (GDI) for G??i/o subunits, stabilising them in their GDP-bound state. It is regulated by upstream kinases such as CDK1 and by GPCR signaling pathways. During mitosis, cortical GPSM1 recruits the polarity proteins GPSM2 (LGN) and NuMA, which anchor dynein/dynactin motor complexes that exert pulling forces on astral microtubules, thereby orienting the mitotic spindle. This pathway is essential for asymmetric cell division and cell fate determination. Additionally, GPSM1 modulates downstream effectors including adenylyl cyclase and ion channels, linking mitotic control to broader G-protein signaling networks.
In the context of HAP1 CML cells, GPSM1 knockout provides an opportunity to examine the intersection of G-protein signaling and leukemic cell biology. Disruption of GPSM1 may alter sensitivity to tyrosine kinase inhibitors such as imatinib, enabling drug target validation studies. The near-haploid nature of the host cell line enhances the penetrance of the knockout phenotype, facilitating robust readouts in assays for spindle orientation defects, cell cycle progression, and signaling pathway activity.
These polyclonal knockout cells are suitable for diverse experimental workflows, including immunofluorescence and live-cell imaging to visualise spindle apparatus dynamics, co-immunoprecipitation coupled with western blotting to probe GPSM1 interaction partners like G??i/o, LGN, and NuMA, and flow cytometry for cell cycle analysis. RT-qPCR can be used to profile transcriptional consequences of GPSM1 loss, while imatinib sensitivity assays may reveal drug response alterations. They also serve as a genetic background for modifier screens to identify additional regulators of mitosis and G-protein signaling. For further information and custom inquiries, please contact Ascent Research.
