The GRB7 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population disrupted for GRB7 loss-of-function. Without single-cell cloning, the heterogeneous pool preserves population-level variation while abrogating GRB7 expression. This polyclonal knockout strategy avoids clonal biases, ensuring that functional studies reflect collective cellular responses rather than isolated clonal effects. The model enables robust investigation of GRB7-dependent signaling and phenotypic consequences.
The Jurkat cell line is an immortalized human T lymphocyte line originating from T cell acute lymphoblastic leukemia, widely used for studying TCR signaling and leukemia. With PTEN and INPP5D mutations causing constitutive PI3K/AKT activity, it serves as a model for RTK and adaptor protein signaling. Genetic manipulation and suspension culture are straightforward, facilitating knockout generation for pathway analysis.
GRB7 adaptor protein transduces signals from activated RTKs (EGFR, HER2, PDGFR) and FAK. It binds phosphotyrosines via its SH2 domain, recruiting Shc, Grb2, SOS, and p130Cas to activate PI3K/AKT and MAPK/ERK pathways, thereby regulating migration, proliferation, and survival. GRB7 overexpression is implicated in breast, gastric, and esophageal squamous cell carcinomas, enhancing metastatic potential. Key signaling axes include GRB7??FAK??PI3K/AKT and GRB7??Shc??Grb2??SOS??Ras??ERK.
In Jurkat T cells, GRB7 is engaged in TCR signaling and integrin-mediated adhesion and migration, processes essential for lymphocyte trafficking and leukemic dissemination. Ablation of GRB7 permits thorough analysis of its effects on TCR-induced phosphorylation of AKT and ERK1/2, FAK activation, and cytoskeletal rearrangement. This model elucidates the contribution of adaptor proteins to hematopoietic malignancy signaling and cross-talk between integrin and growth factor pathways, providing a physiologically relevant system for mechanistic investigations.
These polyclonal knockout cells are designed for dissecting GRB7 roles in TCR signaling, integrin-mediated migration in leukemia, and metastasis. Standard assays include Western blotting, RT-qPCR, Transwell migration, invasion, adhesion, and proliferation. Phospho-signaling analysis of pAKT and pERK can be performed via flow cytometry or Western blotting. The cells are a valuable resource for drug discovery targeting GRB7 pathways and for validating pharmacological inhibitors. For further information, please contact Ascent Research.
