The GYS1 Knockout DLD-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population generated from the DLD-1 human colorectal adenocarcinoma cell line, with targeted disruption of the GYS1 gene. This loss-of-function model delivers a heterogeneous cellular background for investigating glycogen synthase 1 function, avoiding the limitations of single-cell clonal expansion.
The parental DLD-1 line is a widely used human colorectal adenocarcinoma model, originally isolated from a male patient, displaying epithelial morphology. It is a standard tool in colorectal cancer research, particularly for studies of tumor metabolism, signaling transduction, and therapeutic interventions.
The gene product GYS1, glycogen synthase 1, is the rate-limiting enzyme for glycogen synthesis, catalyzing the transfer of glucose from UDP-glucose to a growing glycogen chain initiated by glycogenin (GYG1). The enzyme is regulated by phosphorylation: GSK3??, AMPK, and PKA inactivate it, while PP1-dependent dephosphorylation activates it. Insulin/IGF1 signaling promotes activation via AKT-mediated inhibition of GSK3??. GYS1 interacts with GYG1, PPP1R3C, GBE1, laforin, and malin, and directs glycogen production, thereby modulating glucose-1-phosphate, glucose-6-phosphate, and cellular energy reserves.
In the DLD-1 colorectal adenocarcinoma setting, GYS1 disruption permits dissection of glycogen synthesis contributions to cancer cell metabolism. Colorectal tumors often exhibit altered energy storage and metabolic reprogramming, making this knockout model valuable for probing GYS1-driven effects on proliferation, survival, and adaptation. It is particularly suited to study metabolic dependencies, potential roles in cachexia, and glycogen storage disease mechanisms.
This polyclonal knockout cell population supports diverse experimental applications, including cellular metabolism studies, cancer metabolic reprogramming analysis, and glycogen storage disease modeling. Common assay modalities include PAS staining for glycogen quantitation, western blotting for GYS1 and phospho-GYS1, glucose uptake assays, Seahorse metabolic flux profiling, proliferation assays (MTT/BrdU), colony formation tests, and RT-qPCR for metabolic gene expression. For additional technical details or ordering assistance, please contact Ascent Research.
