The H2BC12L Knockout HEK293T Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal population of human embryonic kidney HEK293T cells with targeted disruption of the H2BC12L gene. This gene encodes the canonical histone H2B protein, a core component of the nucleosome essential for chromatin structure and genome-wide transcriptional regulation. The polyclonal format provides a heterogeneous pool of knockout cells, enabling the study of H2B loss-of-function without clonal selection bias. This model is designed for investigating histone variant biology and nucleosome dynamics in a well-characterized human cell background.
The host cell line, HEK293T, is a widely used derivative of the HEK293 human embryonic kidney epithelial cell line. These cells stably express the SV40 large T antigen, which promotes episomal replication of plasmids containing the SV40 origin of replication, making them particularly suitable for high-level recombinant protein expression and lentiviral production. Their epithelial origin and robust growth characteristics also render them a valuable model for studying signaling pathways, protein interactions, and cellular processes such as chromatin organization and transcriptional control.
H2BC12L encodes the canonical histone H2B protein, a core component of nucleosomes. Its expression is tightly regulated during the cell cycle by E2F transcription factors, NF-Y, and the replication-coupled expression machinery. H2B assembles with histones H2A, H3, and H4 to form the nucleosome, interacting extensively with histone chaperones such as NAP1 and FACT, ATP-dependent chromatin remodelers like the SWI/SNF complex (containing BRG1), and histone-modifying enzymes including acetyltransferases (e.g., p300) and deacetylases (HDACs). Disruption of H2BC12L leads to nucleosome imbalance, chromatin decompaction, and dysregulated RNA polymerase II transcription, potentially affecting DNA replication and repair.
In the HEK293T cellular context, H2BC12L knockout generates a powerful tool for dissecting histone H2B function in human epithelial cells. The resulting chromatin decompaction and altered gene expression profiles provide a platform to investigate epigenetic mechanisms underlying cellular identity and proliferation. This model is particularly relevant for research into chromatinopathies and cancer, where histone mutations and imbalances are increasingly recognized as drivers of oncogenesis and developmental abnormalities.
The H2BC12L knockout cells support a broad array of experimental approaches, including western blotting for histone H2B levels, chromatin immunoprecipitation (ChIP) for histone modifications, MNase-seq and ATAC-seq for nucleosome positioning and chromatin accessibility, and RNA-seq for transcriptome analysis. Chromatin fractionation and co-immunoprecipitation assays enable biochemical characterization of histone complexes. These applications facilitate studies in epigenetic regulation, nucleosome dynamics, and cancer epigenetics. For additional information, technical support, or ordering, please contact Ascent Research.
