The H4C1 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population for loss-of-function studies of the H4C1 gene in the A2780 human ovarian carcinoma epithelial line. This polyclonal pool preserves population-level heterogeneity and enables functional analysis without clonal artifacts. The cells are derived from the A2780 host and are suitable for investigating histone biology and chromatin dynamics.
The A2780 host cell line was established from an untreated ovarian carcinoma patient and is a widely used model for ovarian cancer research. These epithelial cells are characteristic of high-grade serous carcinoma and are extensively employed to study proliferation, drug sensitivity, and DNA repair. This clinically relevant background is ideal for exploring chromatin-related mechanisms in ovarian cancer.
H4C1 encodes replication-dependent histone H4, a core nucleosomal protein essential for chromatin structure and DNA packaging. Its expression is primarily regulated during S-phase by E2F transcription factors, NF-Y, Oct-1, HINFP, and cyclin E/CDK2. Histone H4 is assembled into nucleosomes with histones H2A, H2B, and H3, assisted by chaperones ASF1A, ASF1B, CAF-1, and HIRA, and remodeled by SWI/SNF and ISWI complexes. H4C1-mediated chromatin assembly governs global chromatin condensation and transcriptional regulation. Disruption of H4C1 impairs nucleosome formation, deregulates gene expression, and compromises DNA repair, leading to genomic instability.
In A2780 ovarian cancer cells, H4C1 knockout heightens genomic instability and disrupts cell cycle progression, offering a model to study chromatin dysfunction in oncogenic transformation. The polyclonal knockout allows assessment of collective effects on DNA damage response and epigenetic regulation, and facilitates exploration of therapeutic liabilities such as sensitivity to DNA-damaging agents or epigenetic inhibitors.
This knockout product supports research in epigenetics, chromatin biology, DNA damage response, cancer biology, and cell cycle regulation. Compatible assays include Western blotting for histone H4, RT-qPCR, ChIP-qPCR for histone modifications, immunofluorescence for chromatin structure, flow cytometry for cell cycle, RNA-seq, and apoptosis assays. For further details or customized requests, please contact Ascent Research.
