The HAVCR1 Knockout HeLa Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population engineered to disrupt the HAVCR1 gene in the HeLa cervical adenocarcinoma cell line. This polyclonal model provides a versatile loss-of-function system for interrogating TIM-1 (HAVCR1) biological functions, reflecting heterogeneous editing outcomes typical of polyclonal populations without single-cell cloning.
HeLa cells, derived from a cervical adenocarcinoma of Henrietta Lacks, are an immortalized human cell line widely employed in cancer biology, virology, and signal transduction research. Their robust proliferation and extensive characterization make them a standard host for gene perturbation studies.
The HAVCR1 gene encodes TIM-1, a phosphatidylserine receptor that mediates phagocytosis of apoptotic cells and serves as a cellular entry receptor for hepatitis A virus and other enveloped viruses. Ligand engagement triggers intracellular signaling through PI3K/AKT and NF-??B pathways. Upstream regulators include IL-4, STAT6, NF-??B, and TGF-??, while downstream targets encompass AKT, NF-??B, MAPK, and BCL2. TIM-1 functionally interacts with phosphatidylserine, forms homodimers, and associates with ITGB1, LCK, and ZAP70. Representative pathway components include HAVCR1??PI3K??AKT, HAVCR1??NF-??B, and HAVCR1??BCL2 family, collectively modulating cell survival, proliferation, and immune responses.
In the HeLa context, HAVCR1 knockout impairs viral entry and apoptotic cell clearance, and potentially alters signaling networks that intersect with T-cell co-stimulatory pathways. This model is particularly valuable for dissecting viral entry mechanisms and apoptosis regulation in a well-characterized human cancer cell background, despite the absence of an endogenous immune repertoire.
Research applications include viral infection assays for hepatitis A and other HAVCR1-dependent viruses, apoptosis analysis using Annexin V/PI staining, phosphatidylserine binding assessed by flow cytometry, and RT-qPCR or Western blotting for downstream targets such as AKT, NF-??B, and BCL2. This knockout product is suitable for studies on immune checkpoint biology, kidney injury, and cancer cell survival. For additional information or custom requests, please contact Ascent Research.
