The HAVCR1 Knockout PaTu 8988t Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human PaTu 8988t pancreatic cancer cell line, designed for targeted disruption of the HAVCR1 gene. This polyclonal format provides a heterogeneous pool of knockout cells, enabling robust loss-of-function studies without the limitations of clonal selection.
The parental PaTu 8988t cell line is an epithelial pancreatic ductal adenocarcinoma model isolated from a primary pancreatic tumor. This line retains characteristic features of pancreatic cancer, including uncontrolled proliferation, invasive capacity, and a molecular profile reflective of ductal origin. It provides a clinically relevant cellular context for investigating tumor biology, signal transduction, and therapeutic responses specific to pancreatic cancer.
HAVCR1 encodes the transmembrane glycoprotein TIM-1, which functions both as a co-stimulatory receptor on T cells and as a phosphatidylserine receptor for the clearance of apoptotic cells. TIM-1 is transcriptionally regulated by IL-4 and IL-13 through STAT6. Upon ligand engagement??such as phosphatidylserine, the p85 subunit of PI3K, or Hepatitis A virus capsid??it activates downstream PI3K/AKT and MAPK signaling cascades. TIM-1 also interacts with TIM-4 to modulate immune cell communication. Consequently, knockout of HAVCR1 abolishes TIM-1-mediated signaling, leading to impaired activation of PI3K, AKT, and MAPK, and reduced cytokine production, including IL-4 and IFN-??.
In the PaTu 8988t pancreatic cancer background, the HAVCR1 knockout polyclonal cells offer a powerful tool to dissect the role of TIM-1 in tumor?Cimmune interactions and cancer cell signaling. Although best characterized in lymphocyte biology, TIM-1 expression in epithelial cancers such as pancreatic ductal adenocarcinoma suggests potential functions in tumor cell survival, migration, or immune evasion. The use of a polyclonal knockout population avoids clonal artifacts and provides a more genetically diverse model for studying these pathways.
These cells are applicable to a wide range of experimental techniques, including Western blotting, RT-qPCR, flow cytometry, immunofluorescence, co-immunoprecipitation, apoptosis assays, migration/invasion studies, and drug sensitivity testing. They support research into TIM-1 biology in immune regulation, viral entry mechanisms, and cancer immune surveillance. For further information regarding this product, please contact Ascent Research.
