The HAVCR1 Knockout SK-OV-3 Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal knockout cell population generated from the SK-OV-3 human ovarian cancer cell line. This population carries a targeted disruption of the HAVCR1 gene, which encodes the phosphatidylserine receptor and immune checkpoint protein TIM-1. The polyclonal format reflects a heterogeneous mixture of edited cells, each harboring individual CRISPR/Cas9-mediated gene disruptions, suitable for studying loss-of-function effects without clonal selection artifacts.
The parental SK-OV-3 cell line (ATCC HTB-77) is an epithelial ovarian adenocarcinoma line originally isolated from the ascites of a 64-year-old female patient. These cells are well-characterized for their resistance to tumor necrosis factor (TNF) and various cytotoxic drugs, and they express mutant p53, EGFR, and HER2/neu. Such features make SK-OV-3 a robust model for investigating ovarian cancer progression, chemoresistance, and oncogenic signaling networks.
At the molecular level, HAVCR1 (TIM-1) is a phosphatidylserine receptor that mediates phagocytosis of apoptotic cells and immune regulation. Ligand engagement triggers Src/PI3K/AKT signaling, leading to NF-??B and STAT3 activation and subsequent cytokine release (e.g., IL-6, TNF-??). It also functions as a viral entry receptor for hepatitis A virus, Ebola virus, and SARS-CoV-2, interacting with viral capsid or glycoprotein components. Upstream modulators include TIM-4, IL-4, and TGF-??, while downstream effectors encompass AKT, ERK, Bcl-2 family proteins, and the pro-inflammatory transcription factor NF-??B.
In the context of SK-OV-3 ovarian cancer cells, HAVCR1 loss provides a powerful system to dissect its contributions to tumor cell biology. Given the parental cell??s drug-resistant phenotype and expression of oncogenic drivers, ablation of TIM-1 enables exploration of its role in chemoresistance, apoptosis regulation, and immune evasion mechanisms. The knockout cells may reveal how HAVCR1 signaling intersects with EGFR/HER2 pathways and mutant p53 networks, potentially influencing metastatic properties and response to TNF-mediated cytotoxicity.
These polyclonal knockout cells are suited for phagocytosis assays, viral entry inhibition studies, cytokine ELISA, Western blotting of downstream signaling targets, and co-immunoprecipitation to analyze TIM-4 or PI3K interactions. Additional applications include migration/invasion assays, drug sensitivity testing, and biomarker discovery in ovarian cancer research. For further information, contact Ascent Research.
