The HBP1 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population with targeted disruption of the HBP1 gene. This product provides a heterogeneous loss-of-function model to study the roles of the HBP1 transcription factor in a T lymphocyte context. The polyclonal nature ensures representation of diverse editing outcomes, facilitating functional genomics studies without monoclonal selection.
Jurkat E6-1 is a human T lymphoblastoid cell line derived from acute T cell leukemia, widely used to investigate T cell receptor signaling, immune response, apoptosis, and proliferation. Its robust growth and well-characterized signaling pathways make it an ideal host for gene knockout studies relevant to T cell biology and leukemogenesis.
HBP1 is a transcriptional repressor and tumor suppressor that binds DNA via its HMG-box domain. It is a downstream effector of Wnt/??-catenin signaling: upon WNT3A stimulation, ??-catenin/TCF4 complexes activate HBP1 expression. HBP1 then represses oncogenes such as CCND1 (cyclin D1) and MYC by recruiting cofactors including RB1, p130, SIN3A, HDAC1, and HDAC2. This repression controls cell cycle progression and apoptosis. HBP1 also integrates signals from TGF-?? and MAPK14 pathways and regulates genes like CDKN1A and BAX, placing it at the intersection of multiple tumor-suppressive networks.
In Jurkat T-ALL cells, disruption of HBP1 removes a critical growth-suppressive brake, potentially enhancing proliferation and survival. This knockout model permits investigation of how loss of HBP1-mediated transcriptional repression contributes to T cell malignant transformation, particularly through deregulation of the Wnt/??-catenin?CCCND1 axis. The polyclonal population mirrors the genetic heterogeneity of tumors, enabling robust assessment of HBP1 function.
This product supports a range of experimental approaches: RT-qPCR and Western blot for gene/protein expression; ChIP-qPCR to assess HBP1 binding to CCND1 promoter; cell proliferation assays (MTT, BrdU); and flow cytometry for cell cycle profiling. Luciferase reporters can measure HBP1 transcriptional activity. These cells are valuable for cancer biology, transcriptional regulation, and leukemia research. For further technical details and ordering information, please contact Ascent Research.
