HCK Knockout THP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population of THP-1 cells harboring targeted disruption of the HCK gene. This heterogeneous knockout pool facilitates loss-of-function studies while avoiding clonal selection biases, making it suitable for population-level assays that require biological variability.
The THP-1 cell line, derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia, is a classical model for monocyte/macrophage differentiation. Upon phorbol ester or cytokine stimulation, THP-1 cells differentiate into macrophage-like cells, exhibiting adhesion, phagocytosis, and secretion of inflammatory mediators. This background is ideal for dissecting myeloid signaling and innate immunity.
HCK is a myeloid-dominant Src family tyrosine kinase that transduces signals from integrin receptors, Fc?? receptors, TLRs, and cytokine receptors (G-CSF, GM-CSF). Once activated, HCK phosphorylates downstream targets such as Syk, PI3K, Vav, and paxillin, triggering MAPK cascades, NF-??B, and STAT3. These pathways collectively regulate phagocytosis, adhesion, and pro-inflammatory cytokine production, including TNF-?? and IL-6.
Disruption of HCK in THP-1 cells attenuates integrin-mediated adhesion, Fc receptor-dependent phagocytosis, and TLR-induced NF-??B signaling, leading to reduced cytokine secretion. This knockout model thus enables precise interrogation of HCK??s contributions to myeloid leukemia signaling, inflammatory disease mechanisms, and Src kinase pathway function. It also serves as a valuable system for screening Src kinase inhibitors and evaluating their effects on innate immune responses.
Researchers can employ this polyclonal product in a variety of assays: Western blotting and RT-qPCR for knockout verification, phagocytosis assays with fluorescent particles, cytokine ELISA (e.g., TNF-??, IL-6), transwell migration, phospho-kinase arrays, and co-immunoprecipitation of ITAM-containing receptor complexes. Flow cytometry can assess activation markers and differentiation status. Applications range from basic inflammation research to drug discovery for myeloid malignancies. For technical inquiries or custom orders, contact Ascent Research.
