HDAC4 Knockout DLD-1 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Large intestine (colon)
Disease:
Adenocarcinoma

Datasheet

CRISPR/Cas9-edited polyclonal HDAC4 knockout DLD-1 cells provide a loss-of-function model in a human colorectal adenocarcinoma background. HDAC4 is a class IIa histone deacetylase that represses transcription by deacetylating histones and transcription factors such as MEF2 and p53, and its activity is regulated by CaMKII/PKD phosphorylation and 14-3-3-mediated nuclear export. These polyclonal knockout cells are ideal for investigating HDAC4-dependent epigenetic regulation, colorectal cancer biology, and drug responses. Applications include proliferation and apoptosis assays, ChIP-qPCR for histone acetylation changes, and screening of HDAC inhibitors in colorectal cancer research.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

DLD-1

Age

Adult

Gene Name

HDAC4

Gene Identifier

NCBI Gene ID 9759

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The HDAC4 Knockout DLD-1 Polyclonal Cells product consists of a polyclonal population of DLD-1 human colorectal adenocarcinoma cells (Homo sapiens) engineered via CRISPR/Cas9-mediated disruption of the HDAC4 gene.

This targeted gene disruption generates a loss-of-function model suitable for studying the biological roles of HDAC4 in a colorectal cancer context.

The polyclonal format avoids biases inherent to single-cell clones and provides a representative distribution of knockout alleles within the cell pool.