The HDAC8 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with targeted disruption of the HDAC8 gene in the HGC-27 gastric carcinoma cell line, providing a loss-of-function model to study HDAC8-dependent pathways. The polyclonal format enables pooled functional analyses and preserves genetic diversity, avoiding clonal artifacts.
The parental HGC-27 cell line is a poorly differentiated gastric adenocarcinoma line originally isolated from a lymph node metastasis. These cells exhibit an epithelial morphology and are widely employed as a model for metastatic gastric carcinoma, particularly for studying invasion and migration mechanisms. HGC-27 cells harbor genetic and phenotypic features representative of aggressive gastric cancer, making them a relevant host for evaluating the functional consequences of HDAC8 knockout in a metastatic context.
HDAC8 is a class I histone deacetylase that removes acetyl groups from histones H3 and H4 and from non-histone proteins including p53 (at K382), cortactin, and SMC3. Histone deacetylation compacts chromatin and silences tumor suppressors; p53 deacetylation inactivates its transcriptional activity; and cortactin deacetylation facilitates invadopodia formation and invasion. HDAC8 is regulated by upstream signaling through CREB, E2F1, STAT3, and PI3K/AKT, and interacts with cohesin complex components SMC3 and RAD21 as well as CBP/p300. Downstream, HDAC8 signaling converges on the p53 (p21, BAX), STAT3 (cyclin D1, Bcl-2), and Wnt/??-catenin (??-catenin, TCF/LEF) pathways, thereby influencing cell cycle, apoptosis, and motility.
In HGC-27 gastric cancer cells, HDAC8 promotes proliferation, migration, and metastasis by suppressing tumor suppressors and facilitating invasion. This polyclonal knockout model allows unbiased assessment of HDAC8 loss on these phenotypes, with expected derepression of tumor suppressors and reduced oncogenic signaling, providing a robust system to dissect HDAC8??s contributions to gastric adenocarcinoma progression.
This HDAC8 Knockout HGC-27 Polyclonal Cells product is designed for research applications such as elucidating epigenetic mechanisms in gastric cancer, screening HDAC8-selective inhibitors like PCI-34051, and functional studies of HDAC8 in cell cycle, apoptosis, and metastasis. Compatible assays include western blotting, RT-qPCR, transcriptomics, ChIP-qPCR for histone acetylation (e.g., H3K27ac), immunofluorescence, flow cytometry for cell cycle and apoptosis, wound healing, transwell invasion, colony formation, co-immunoprecipitation, and drug sensitivity testing. For further information, please contact Ascent Research.
