HMGA2 Knockout HEK293T Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Kidney

Datasheet

The HMGA2 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-mediated HMGA2-knockout heterogeneous polyclonal population in the HEK293T background. HMGA2 is a chromatin architectural transcription factor regulated by let-7 microRNA, TGF-??, and Wnt ligands, interacting with RB1, NF-??B, and SMAD2/3, and driving expression of CCNA2, CDC25A, and TWIST1. This model enables investigation of proliferation, cell cycle progression, migration, and oncogenic transformation, with applications including flow cytometric cell cycle analysis, wound healing assays, and soft agar colony formation, facilitating research into HMGA2??s role in tumorigenesis and signaling networks.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

HEK293T

Sex of Donor

Female

Age

Fetus

Derived From Site

Fetal kidney

Gene Name

HMGA2

Gene Identifier

NCBI Gene ID 8091

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

DMEM

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The HMGA2 Knockout HEK293T Polyclonal Cells product provides a CRISPR/Cas9-edited polyclonal knockout cell population in which the HMGA2 gene has been disrupted, creating a heterogeneous loss-of-function model for dissecting HMGA2-dependent cellular mechanisms.

This population, derived from bulk editing without single-cell cloning, preserves biological variability and avoids clonal selection artifacts, making it an ideal tool for robust functional genomic studies in a widely used epithelial host background.