HMGXB3 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the HeLa cervical adenocarcinoma line. This product provides targeted disruption of HMGXB3, a gene encoding a putative chromatin-associated transcription factor with an HMG-box domain. The polyclonal pool comprises a heterogeneous mix of gene-edited cells, offering a practical loss-of-function model for population-level studies without clonal selection. It is well-suited for investigating HMGXB3 function in chromatin biology and cancer.
The host HeLa cell line is an HPV18-positive epithelial adenocarcinoma model in which the viral oncoproteins E6 and E7 inactivate p53 and Rb, respectively. This genetic background promotes uncontrolled proliferation and is widely used in cancer research, including studies of chromatin dynamics and signaling. The HPV18-driven transformation provides a relevant context for examining how chromatin-associated factors contribute to oncogenic phenotypes.
HMGXB3 is predicted to act as a chromatin organizer and transcriptional regulator, binding DNA via its HMG-box domain and interacting with TCF/LEF transcription factors and chromatin remodelers. It is implicated in Wnt signaling, where it may function downstream of Wnt ligands and ??-catenin to modulate expression of genes governing proliferation and differentiation. Knockout of HMGXB3 disrupts these interactions, enabling dissection of its role via ChIP-seq and RNA-seq.
In the HeLa background, HMGXB3 loss may compromise Wnt-driven proliferative and migratory programs, as the protein is thought to facilitate chromatin-level regulation required for oncogenic gene expression. This model can reveal context-specific dependencies between chromatin-associated factors and oncogenic signaling, particularly given p53/Rb deficiency. Thus, the HMGXB3 knockout population serves as a valuable tool for exploring cancer-relevant mechanisms.
Applications include Western blotting for knockout confirmation, RT-qPCR for target gene analysis, and functional assays such as proliferation and migration/invasion studies. Transcriptomic (RNA-seq) and chromatin binding (ChIP-seq) analyses can further elucidate HMGXB3??s regulatory network. The polyclonal format supports population-level readouts and drug response profiling. Researchers in Wnt signaling, chromatin remodeling, or functional genomics will find this product highly useful. For further details, please contact Ascent Research.
