The HNRNPA2B1 Knockout HEK293T Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population designed for loss-of-function studies of the RNA-binding protein HNRNPA2B1. Through targeted gene disruption, this model abolishes endogenous HNRNPA2B1 expression, enabling precise dissection of its post-transcriptional regulatory roles without confounding wild-type activity.
The parental HEK293T cell line, derived from human embryonic kidney and immortalized with adenovirus 5 DNA, stably expresses the SV40 large T antigen, conferring exceptional transfectability and viral production capacity. These features make it an optimal host for analyzing gene function across diverse experimental paradigms, including transient expression and signaling assays.
HNRNPA2B1 is a nucleocytoplasmic shuttling protein that regulates pre-mRNA alternative splicing, m6A-dependent mRNA nuclear export via XPO1, and cytoplasmic translation and stability. It interacts with spliceosome components (U1/U2 snRNPs), hnRNPA1, hnRNPC, and the METTL3/METTL14 m6A methyltransferase complex. Upstream regulators include transcription factors MYC and E2F1, as well as heat shock and oxidative stress. Downstream targets encompass proliferation (MYC, CCND1), apoptosis (BCL2L1), and inflammatory (TNF) mRNAs, along with telomeric TERRA RNA. Additionally, HNRNPA2B1 senses viral nucleic acids, activating cGAS-STING-mediated innate immunity.
Ablation of HNRNPA2B1 in HEK293T cells eliminates a key node in RNA metabolism and innate immune pathways, providing a defined system for functional dissection. The polyclonal population minimizes clonal biases, and the cells’ high transfectability facilitates rescue experiments.
Researchers can apply this polyclonal knockout model to transcriptome-wide investigations of splicing and m6A modifications using RNA-seq and MeRIP-seq, cancer cell proliferation and migration assays, and innate immune studies involving viral infection and interferon induction readouts. Key techniques include RNA immunoprecipitation, co-immunoprecipitation, Western blotting, and immunofluorescence for analyzing protein?CRNA interactions and subcellular localization. Neurodegenerative disease research focusing on stress granule dynamics also benefits from this resource. For further technical support, please contact Ascent Research.
