The HNRNPA3 Knockout HEK293T Polyclonal Cells provide a CRISPR/Cas9-edited polyclonal population with disrupted HNRNPA3 expression. This mixed pool of HEK293T cells harbors diverse loss-of-function mutations, offering a robust model to study HNRNPA3 roles in RNA metabolism while minimizing clonal artifacts. The knockout eliminates full-length HNRNPA3 protein, enabling dissection of its post-transcriptional regulatory functions.
The HEK293T host cell line, a derivative of human embryonic kidney cells expressing SV40 large T-antigen, provides high transfection efficiency and supports episomal plasmid replication. Widely used for protein expression and viral packaging, its robust growth characteristics make it ideal for studying RNA-binding proteins. The HNRNPA3 knockout in this background allows interrogation of splicing and transport mechanisms without lineage-specific complexities.
HNRNPA3 is an RNA-binding protein central to pre-mRNA processing and alternative splicing, regulated by TP53 and stress signals through phosphorylation and methylation. It interacts with spliceosomal components including HNRNPA2B1, HNRNPC, SRSF1, and TDP-43. Through these interactions, it modulates alternative splicing of targets like CD44 and SMN and facilitates mRNA export via NXF1. Disruption perturbs splice site selection, alters isoform profiles, and compromises mRNA stability, leading to widespread gene expression changes.
HNRNPA3 deficiency in HEK293T creates a model for aberrant RNA processing in cancer and neurodegeneration, partly through disrupted TDP-43 interaction and stress granule dynamics. The knockout enables analysis of how HNRNPA3 loss alters stress responses, redistributes interacting factors, and reshapes transcript isoform landscapes. Additionally, its viral packaging utility extends to studying HNRNPA3 in viral RNA metabolism and host-pathogen interactions.
This polyclonal knockout supports diverse assays: Western blotting and RT-qPCR for confirming disruption and splice variant quantification; RNA-seq for transcriptome-wide splicing analysis; RNA immunoprecipitation (RIP) for mapping RNA-protein interactions with HNRNPA3, HNRNPA2B1, or SRSF1; and immunofluorescence for localization studies to monitor stress granule dynamics. These tools facilitate investigations into cancer and neurodegenerative disease mechanisms as well as therapeutic target evaluation. For additional product information and technical support, please contact Ascent Research.
