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Home / Products / Genome-edited Cells / Knockout Cell Lines / HNRNPA3 Knockout Hela Polyclonal Cells

Cat. No. ARG37549

HNRNPA3 Knockout Hela Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Uterus (cervix)
Disease:
Adenocarcinoma

Datasheet

The HNRNPA3 Knockout HeLa Polyclonal Cells provide a CRISPR/Cas9-edited heterogeneous population of HeLa cells with disrupted HNRNPA3 function. This loss-of-function model targets the RNA-binding protein HNRNPA3, which regulates alternative splicing, mRNA transport, and telomere maintenance through interactions with factors such as hnRNP A1, POT1, and TPP1, and is responsive to MYC signaling. These polyclonal knockout cells are ideal for investigating telomere biology, RNA metabolism, and cancer mechanisms. They enable assays like qPCR for splicing changes, telomere length measurement, and protein expression analysis, supporting research in oncology, neurodegeneration, and drug target discovery.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

HeLa

Sex of Donor

Female

Age

31 years

Gene Name

HNRNPA3

Gene Identifier

NCBI Gene ID 220988

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

MEM (with NEAA)

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The HNRNPA3 Knockout HeLa Polyclonal Cells product consists of a heterogeneous population of HeLa cells carrying CRISPR/Cas9-mediated disruptions in the HNRNPA3 gene. This polyclonal knockout pool provides a loss-of-function model for investigating the roles of heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3) in RNA metabolism and telomere biology.

HeLa cells are an immortalized human cervical adenocarcinoma line positive for HPV-18, extensively used in cancer research, cell cycle studies, and viral oncology. Their transformed phenotype and ease of genetic manipulation make them a suitable host for generating polyclonal knockout populations to explore gene function in a disease-relevant setting.

HNRNPA3 is an RNA-binding protein that regulates alternative splicing, mRNA transport, and telomere maintenance. Its function is influenced by cellular stress and MYC oncogenic signaling. HNRNPA3 interacts with hnRNP A1, hnRNP A2/B1, RNA polymerase II, and spliceosomal components. It binds downstream targets including telomeric repeat-containing RNA (TERRA), BCL2L1 pre-mRNA, and immune-related transcripts. In telomere biology, HNRNPA3 associates with POT1 and TPP1, connecting it to the telomerase complex and RNP granules. Disruption of HNRNPA3 in HeLa cells may impair telomeric RNA processing and alter splicing of apoptosis and cell cycle regulators.

The HeLa cell background, with HPV-18-mediated inactivation of p53 and Rb, offers a unique platform to study HNRNPA3 function in the context of viral transformation. The polyclonal knockout format avoids clonal selection bias and enables examination of population-level responses. This model is particularly suited for probing the role of HNRNPA3 in telomere integrity and splicing-dependent regulation of proliferation and survival in cancer.

These knockout cells can be utilized in RNA immunoprecipitation, RT-qPCR for splicing isoform analysis, telomere length measurement, immunofluorescence, western blotting, and cell cycle assays. They support research in RNA metabolism, telomere biology, cancer progression, and neurodegenerative disease modeling, and are valuable for drug target discovery. For technical questions or customized protocols, please contact Ascent Research.