The HNRNPAB Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from HEK293T cells, providing a loss-of-function model for HNRNPAB research. This heterogeneous pool of cells, each carrying distinct gene disruptions, allows population-level studies without clonal selection bias. The polyclonal format is ideal for functional genomics applications focused on RNA biology, telomere maintenance, and DNA damage response.
The host HEK293T cell line is a human embryonic kidney derivative expressing the SV40 large T antigen, which enables high-level transient protein expression and efficient lentiviral packaging. Widely used for transfection-based experiments, HEK293T exhibits rapid growth and supports episomal replication of plasmids with SV40 ori. Although the large T antigen inactivates p53 and Rb, this background remains a versatile platform for studying cell-autonomous pathways such as RNA metabolism and genome stability.
HNRNPAB encodes an RNA-binding protein involved in pre-mRNA processing, alternative splicing, and translational control. It interacts with telomere components TERT, POT1, TRF1, and TRF2 and modulates the non-coding RNA TERRA, critical for telomere integrity. HNRNPAB complexes with U2AF2 and other hnRNPs, and its activity is regulated by ATM/ATR kinases and p53. Downstream, HNRNPAB promotes expression of DNA repair factors and cell cycle regulators. Disruption of HNRNPAB impairs these functions, causing telomere dysfunction, genomic instability, and altered cell cycle progression.
Within the HEK293T context, HNRNPAB knockout offers a tractable model to study how RNA-binding proteins coordinate telomere maintenance and DNA repair. The absence of functional p53 due to SV40 large T antigen allows dissection of p53-independent roles of HNRNPAB in genome surveillance. High transfectability facilitates rescue experiments and structure-function analyses. Researchers can monitor TERRA dynamics, telomere length alterations, and DNA damage foci formation in a readily manipulated human cell system.
Typical applications include RNA-sequencing for splicing analysis, RT-qPCR for target validation, Western blotting, immunofluorescence for ??H2AX foci, cell cycle profiling, and telomere length measurement by qFISH or Southern blotting. This knockout model supports cancer and neurodegenerative disease research where RNA processing and telomere dysfunction intersect. For more information, please contact Ascent Research.
