The HNRNPDL Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the HNRNPDL gene in Jurkat T lymphocytes. This loss-of-function model enables investigation of HNRNPDL’s roles in mRNA processing and post-transcriptional gene regulation. The polyclonal format provides a heterogeneous gene-disrupted pool, facilitating robust functional studies without clonal selection bias. Researchers can use this tool to dissect RNA-binding protein dysfunction in immune cells and related diseases.
The Jurkat host cell line is an immortalized human T lymphocyte model derived from acute T cell leukemia in a 14-year-old male. Widely used in immunology and oncology, Jurkat cells exhibit well-characterized T cell receptor signaling, apoptotic pathways, and leukemic features, offering a relevant background for studying HNRNPDL function in T cell biology and leukemia.
HNRNPDL encodes an RNA-binding protein involved in mRNA splicing, transport, stability, and translational control. It interacts with spliceosome components, RNA helicases, and hnRNP proteins, including HNRNPD (AUF1). Upstream regulators such as the transcription factor MYC and cellular stress signals modulate its activity via post-translational modifications. Downstream, HNRNPDL binds to mRNAs of CCND1, MYC, and BCL2, influencing their expression to regulate cell cycle progression and apoptosis.
In Jurkat cells, HNRNPDL knockout likely disrupts post-transcriptional control of genes critical for T cell function and survival, potentially impairing proliferation and altering stress responses. This disruption may attenuate leukemic phenotypes, making the model valuable for studying RNA-binding protein roles in T cell leukemia and for exploring mechanisms relevant to limb-girdle muscular dystrophy type 1G and neurodegenerative diseases.
This polyclonal knockout model is suitable for applications such as functional genomics, drug target discovery, and mechanistic studies of mRNA regulation. Typical assays include RT-qPCR for gene expression analysis, RNA immunoprecipitation (RIP), western blotting, flow cytometry for cell cycle and apoptosis, RNA sequencing, and cell proliferation assays. By enabling precise interrogation of HNRNPDL function, this product accelerates research into RNA biology in immune cells and cancer. For further information, please contact Ascent Research.
