HP1BP3 Knockout HEK293T Polyclonal Cells are a polyclonal cell population generated by CRISPR/Cas9-mediated disruption of the HP1BP3 gene in the widely used HEK293T host cell line. This product provides a heterogeneous loss-of-function model suitable for studying the functional roles of HP1BP3 in chromatin regulation and DNA damage response. The polyclonal knockout format offers a practical tool for initial screening and functional validation without the need for clonal isolation, enabling robust downstream analyses in a relevant cellular context.
The HEK293T cell line, derived from human embryonic kidney epithelial cells, stably expresses the SV40 large T antigen, which permits episomal replication of plasmids containing the SV40 origin of replication. This feature, combined with its high transfectability and capacity for protein expression and viral production, makes HEK293T a preferred model system for a broad range of molecular and cellular biology applications. The genetic background of HEK293T enables efficient delivery of CRISPR components and supports rapid experimental timelines, making it an ideal host for generating knockout cell pools.
HP1BP3 encodes a chromatin-binding scaffold protein that interacts with heterochromatin protein 1 (HP1) family members, including CBX5 (HP1??), CBX1 (HP1??), and CBX3 (HP1??), to regulate higher-order chromatin structure. HP1BP3 promotes the recruitment of HP1 complexes to specific genomic loci, facilitating heterochromatin formation and transcriptional repression in concert with histone methyltransferases such as SUV39H1 and the H3K9me3 mark. In the DNA damage response, HP1BP3 is phosphorylated by upstream kinases ATM and ATR, leading to its relocalization to damage sites where it helps assemble repair complexes containing factors such as ??H2AX and BRCA1. This dual function positions HP1BP3 at the nexus of epigenetic gene silencing and genome maintenance.
Disruption of HP1BP3 in HEK293T cells provides a valuable loss-of-function model to dissect the interplay between chromatin architecture and DNA damage signaling. Given the high transfectability and well-characterized cell cycle profile of HEK293T, researchers can readily introduce reporters or tagged constructs to monitor HP1BP3-dependent processes. This knockout model is particularly suited for investigating the consequences of impaired heterochromatin integrity on gene expression programs and genomic stability, and for exploring how HP1BP3 coordinates epigenetic silencing with checkpoint signals in a cancer-relevant cellular environment.
Applications include detailed mechanistic studies of chromatin organization, DNA repair foci dynamics, and epigenetic regulation using techniques such as ChIP-qPCR, immunofluorescence, and ??H2AX foci assays. The polyclonal knockout cells can be employed in functional genomics screens, luciferase reporter assays to assess transcriptional activity, and flow cytometry to analyze cell cycle progression defects. Additionally, they serve as a baseline for comparative studies with wild-type or rescue experiments. This product expands the experimental toolkit for scientists investigating chromatin biology, cancer genomics, and therapeutic targets. For more information, please contact Ascent Research.
