The HSPB1 Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population of HT29 human colorectal adenocarcinoma cells with disrupted HSPB1 expression. This loss-of-function model enables investigation of heat shock protein beta-1 (HSPB1) in a colorectal cancer context. The polyclonal format preserves genetic heterogeneity, reflecting tumor diversity in experimental designs. It is suited for researchers requiring robust perturbation of HSPB1 in a well-established colon cancer cell line.
The HT29 cell line, derived from colorectal adenocarcinoma of a 44-year-old Caucasian female, displays epithelial morphology and intestinal marker expression, serving as a standard model for colorectal cancer and intestinal biology. Its tumorigenic capacity and defined signaling landscape make it ideal for dissecting oncogenic pathways and therapeutic responses in colon cancer research.
HSPB1 (Hsp27) is a stress-induced molecular chaperone activated by p38 MAPK, MAPKAPK2, AKT, ERK, and JNK downstream of stimuli like heat shock, oxidative stress, and cytokines (TNF-??, IL-1??). It inhibits apoptosis by sequestering cytochrome c and procaspase-3, blocking caspase activation. Additionally, HSPB1 stabilizes actin filaments and promotes survival via IKK-mediated NF-??B activation. It interacts with CRYAB, HSPB8, and modulates translation through eIF4E. Thus, HSPB1 integrates p38 MAPK stress signaling, AKT survival cascades, and cytoskeletal regulation.
In HT29 cells, HSPB1 contributes to proliferation, chemoresistance (e.g., cisplatin), and metastatic potential. The polyclonal HSPB1 knockout population permits rigorous examination of its roles in apoptosis, drug sensitivity, and invasion. This model is particularly relevant for colorectal cancer research, where HSPB1 overexpression often correlates with poor prognosis and resistance. The heterogeneous knockout pool supports population-based assays, such as drug screening and signaling analyses, bridging in vitro findings with tumor heterogeneity.
Applications include western blotting, RT-qPCR, MTT and Annexin V assays, Transwell migration, wound healing, cisplatin sensitivity testing, phalloidin staining, co-immunoprecipitation, and phospho-p38 ELISA. These tools facilitate studies on stress response, cytoskeletal dynamics, targeted therapy screening, and metastasis mechanisms. The HSPB1 Knockout HT29 Polyclonal Cells are a versatile resource for colorectal cancer and stress biology research. For technical inquiries, contact Ascent Research.
