The HTRA1 Knockout Ca Ski Polyclonal Cells product comprises a polyclonal population of Ca Ski human cervical epidermoid carcinoma cells with CRISPR/Cas9-mediated disruption of the HTRA1 gene. This heterogeneous knockout pool avoids clonal selection, providing a robust model to study loss-of-function phenotypes. Target gene disruption is validated at the genomic and protein levels, ensuring reliable abrogation of HTRA1 function.
The Ca Ski cell line originates from a cervical epidermoid carcinoma metastasis and contains integrated HPV16 genome, rendering it tumorigenic. These HPV16-positive epithelial cells are widely employed in cervical cancer research, particularly for investigating viral oncoprotein interactions with host signaling pathways. The adherent cell line offers a reproducible platform for functional assays in an oncogenic background.
HTRA1 is a secreted serine protease that cleaves substrates including TGF-??1, fibronectin, and amyloid precursor protein, thereby modulating TGF-??, Wnt/??-catenin, and NF-??B signaling. It acts as a tumor suppressor by restraining proliferation and promoting apoptosis. Upstream regulators include TGF-??, EGF, and oxidative stress; frequent epigenetic silencing occurs via promoter methylation. HTRA1 interacts with ??-1-antitrypsin and TGF-?? receptor I, linking it to SMAD2/3, ??-catenin/TCF4, and p65/I??B?? cascades. Knockout disrupts these checkpoints.
In the HPV16-positive Ca Ski background, HTRA1 knockout enables detailed study of tumor suppression mechanisms relevant to cervical cancer progression. Loss of HTRA1 is expected to enhance TGF-?¨Cdependent proliferation, Wnt/??-catenin?Cdriven growth, and NF-??B?Cmediated survival signals. This model also facilitates exploration of extracellular matrix remodeling and its impact on migration and invasion in an epithelial tumor context, aiding identification of therapeutic targets.
Research applications include proliferation (MTT/BrdU), migration/invasion, and apoptosis assays, along with TGF-?? reporter assays and Western blotting for pathway components such as phospho-SMAD2, ??-catenin, and p65. RNA-seq enables transcriptomic profiling, while protease activity assays confirm functional knockout. These cells are also valuable for drug resistance studies. For additional information, please contact Ascent Research.
