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Home / Products / Genome-edited Cells / Knockout Cell Lines / IFI27 Knockout MAC-T Cell Line

Cat. No. ARG0517

IFI27 Knockout MAC-T Cell Line

Product Type:
Genome-edited Cells
Tissue Source:
Breast (mammary gland)
Gene Species:
Bos taurus (Domestic cattle)

Datasheet

The IFI27 Knockout MAC-T Cell Line is a CRISPR/Cas9-edited knockout cell line derived from bovine MAC-T mammary epithelial cells. The target gene, IFI27, encodes an interferon-inducible protein that drives apoptosis and antiviral responses through mitochondrial interactions with Bcl-2 family members and GRIM-19, activating caspases. This model is designed for studying IFI27 function in interferon signaling, apoptosis, and innate immunity within a milk-synthesizing epithelial context. Researchers can perform caspase activity assays, viral replication experiments, and interferon stimulation followed by gene expression profiling.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

MAC-T

Age

Adult

Sex of Donor

Female

Gene Name

IFI27

Gene Alias

Interferon alpha-inducible protein 27; ISG12(A)

Gene Species

Bos taurus (Domestic cattle)

Gene Identifier

NCBI Gene ID 507138

Gene Family

Interferon?stimulated RNA-binding protein family
Culture Conditions

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

Daily monitoring confirms that the cells are free from bacterial, yeast, and fungal contamination.

Mycoplasma testing

Negative for mycoplasma through PCR analysis

Pathogens

Cells tested negative for HIV-1, HBV, and HCV.
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The IFI27 Knockout MAC-T Cell Line is a CRISPR/Cas9-edited knockout cell line providing loss-of-function for the interferon-inducible protein IFI27. This product enables investigation of IFI27-dependent pathways in a physiologically relevant bovine mammary epithelial background. The knockout was generated using CRISPR/Cas9-mediated gene disruption, yielding a stable model for studying apoptosis and antiviral innate immunity.

The host MAC-T cell line is an immortalized bovine mammary epithelial cell line that retains key functions of primary mammary epithelial cells, including the capacity for milk protein synthesis and secretion. Widely used in lactation biology and mastitis research, MAC-T cells provide a relevant in vitro system for examining how mammary epithelial cells respond to cytokines and pathogens.

IFI27 is transcriptionally induced by type I and type II interferons through the JAK-STAT pathway, involving upstream kinases JAK1 and TYK2, transcription factors STAT1, STAT2, and IRF9 (forming the ISGF3 complex), and interferon receptors IFNAR1 and IFNGR1. The IFI27 protein localizes to mitochondria, where it interacts with GRIM-19 (NDUFA13) and Bcl-2 family members including Bcl-2 and Bax, promoting cytochrome c release and activation of Caspase-3 and Caspase-9 to execute apoptosis. Additionally, IFI27 interacts with STAT3 and ISG12 family proteins to contribute to viral replication suppression.

In the MAC-T mammary epithelial context, IFI27 knockout allows dissection of apoptosis and antiviral signaling networks that are critical during mammary gland infection. Mammary epithelial cells frequently encounter viral challenges, and IFI27-mediated cell death may influence pathogen clearance and epithelial barrier integrity. This model is therefore valuable for exploring the intersection of interferon signaling and cell death in bovine mastitis and related inflammatory conditions.

Researchers can utilize this knockout cell line for diverse functional assays, including Annexin V apoptosis staining, Caspase-3/7 activity measurements, and RT-qPCR profiling of antiviral gene expression. Interferon stimulation followed by immunofluorescence for mitochondrial localization enables spatial analysis of IFI27 interactors. Viral replication assays provide direct antiviral assessment, while drug treatment studies support target validation in interferon-related diseases. For further product details or technical assistance, please contact Ascent Research.