IFT80 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population generated to disrupt the IFT80 gene in HAP1 cells. This product offers a mixed pool of gene-edited cells for loss-of-function analysis of IFT80, a core component of intraflagellar transport complex B. The knockout model is suitable for investigating ciliogenesis, cargo trafficking, and associated signaling pathways in a simplified genetic background.
HAP1 is a near-haploid, adherent human cell line derived from the chronic myeloid leukemia line KBM-7. Its haploid karyotype facilitates unambiguous gene disruption studies, as most loci are present in a single copy, eliminating heterozygosity confounds. HAP1 cells are widely adopted for CRISPR-based functional genomics due to their robust growth and compatibility with standard molecular and cellular assays.
IFT80 encodes a subunit of IFT complex B, essential for anterograde transport along ciliary microtubules and required for ciliogenesis. It interacts with IFT20, IFT27, IFT57, IFT74, IFT81, and IFT88 within the complex. The IFT80 gene is regulated by RFX transcription factors and FOXJ1. Disruption of IFT80 compromises ciliary assembly, leading to impaired Hedgehog signaling, where SMO activation and GLI2/SUFU-dependent transcription are particularly affected. IFT80 loss also disturbs Wnt pathway modulation, linking ciliary defects to developmental signaling abnormalities.
In the HAP1 near-haploid context, IFT80 disruption yields a clean loss-of-function system for dissecting ciliary biology. The single-copy nature of IFT80 simplifies genotype-phenotype correlations, making this model ideal for studying skeletal ciliopathies such as Jeune asphyxiating thoracic dystrophy and short-rib polydactyly syndrome. Researchers can directly connect IFT80 deficiency to ciliary ultrastructure defects and aberrant Hedgehog/Wnt signaling outputs.
Applications include ciliopathy disease modeling, IFT mechanistic studies, and drug screening for ciliary function modulators. Compatible assays comprise immunofluorescence for ciliary markers (acetylated tubulin, ARL13B), Western blotting for IFT complex proteins, and RT-qPCR for Hedgehog targets (GLI1, PTCH1). Cell-based functional assays further enable investigation of Wnt-related processes. This product serves as a valuable resource for ciliary signaling and rare disease research. For more information, contact Ascent Research.
