The IGF2BP2 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population, generated by disrupting the IGF2BP2 gene in the HEK293T human embryonic kidney cell line. This model provides a loss-of-function platform for studying the RNA-binding protein IGF2BP2 and its role in post-transcriptional gene regulation, without clonal selection. The polyclonal pool preserves genetic heterogeneity while achieving effective target-gene disruption.
HEK293T cells are an extensively characterized derivative of the HEK293 line, constitutively expressing the SV40 large T antigen. This enables episomal replication of plasmids containing the SV40 origin of replication, making HEK293T a preferred host for high-efficiency transient transfection and lentiviral production. The epithelial origin from human embryonic kidney provides a physiologically relevant context for studying insulin signaling, metabolic pathways, and oncogenic processes, and the line supports robust protein expression and RNA metabolism studies.
IGF2BP2 encodes an mRNA-binding protein that recognizes N6-methyladenosine (m6A) and other cis-elements to regulate stability, localization, and translation. It stabilizes and enhances translation of critical transcripts including MYC, IGF2, CD44, CTNNB1, and LEF1. IGF2BP2 functions downstream of insulin/IGF1 signaling via PI3K?CAKT and mTORC1 pathways, and is modulated by let-7 and miR-196a microRNAs. It interacts with multiple RNA-binding proteins, such as ELAVL1, HNRNPA2B1, YBX1, PABPC1, and eIF4E, forming dynamic ribonucleoprotein complexes that control mRNA fate. Through these interactions, IGF2BP2 promotes cell proliferation, survival, and metabolic reprogramming.
Knockout of IGF2BP2 in HEK293T cells disrupts the post-transcriptional stabilization of oncogenic and metabolic mRNAs, blunting downstream signaling outputs such as MYC-driven proliferation and AKT-mediated metabolic control. This polyclonal knockout model allows interrogation of IGF2BP2-dependent regulation of the insulin?CPI3K?CAKT?CmTOR axis and its cross-talk with mRNA surveillance pathways. Given the HEK293T background??s ease of genetic manipulation and protein expression, the cells serve as a tractable system for dissecting the contribution of IGF2BP2 to mRNA metabolism and signaling networks, and for evaluating compensatory mechanisms that arise upon loss of this hub protein.
This product is ideally suited for a broad range of applications in diabetes research, cancer biology, RNA metabolism studies, and drug target validation. Typical assays include Western blotting and RT-qPCR to assess expression of IGF2BP2 target genes, RNA immunoprecipitation (RIP) for protein?CRNA interaction mapping, RNA stability measurements using actinomycin D chase, and cell proliferation assays. The polyclonal nature also supports RNA-seq and proteomic analyses to globally assess transcriptomic and proteomic changes. For additional product details, validation data, or technical inquiries, please contact Ascent Research.
