The IGF2BP3 Knockout LoVo Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human colorectal adenocarcinoma cell line LoVo, engineered to disrupt the expression of the insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) gene. This pooled knockout model retains genetic heterogeneity while providing a robust loss-of-function system for studying IGF2BP3-dependent processes in colorectal cancer research.
The parental LoVo cell line was originally established from a metastatic lymph node of a 56-year-old male presenting with Dukes’ type C colorectal adenocarcinoma. LoVo cells harbor an activating KRAS G13D mutation and are microsatellite stable (MSS), representing an epithelial model of metastatic colorectal cancer suitable for molecular and pharmacological studies of tumor progression and therapeutic resistance.
IGF2BP3 encodes an oncofetal RNA-binding protein that recognizes N6-methyladenosine (m6A)-modified transcripts, enhancing the stability and translation of target mRNAs including MYC, CD44, and IGF2. It is transcriptionally activated by MYC and CTNNB1 (??-catenin) and post-transcriptionally regulated by LIN28B-mediated suppression of Let-7 miRNAs. IGF2BP3 functions within a network involving interacting partners such as IGF2BP1/2, ELAVL1, DHX9, and YBX1, and its activity converges on pathways including Wnt/??-catenin (via WNT3A, FZD7, DVL2, GSK3B, and TCF4) and PI3K/AKT/mTOR (involving PIK3CA, AKT1, MTOR, RPS6KB1, EIF4EBP1), thereby modulating proliferative and invasive gene expression programs.
In the LoVo background, disruption of IGF2BP3 attenuates the stabilization of oncogenic transcripts and impairs downstream signaling, potentially reducing proliferation, migration, and invasion. The polyclonal knockout population enables assessment of heterogeneous responses to IGF2BP3 loss and is particularly relevant for investigating crosstalk between KRAS-driven MAPK signaling and post-transcriptional regulation in MSS colorectal cancer, as well as exploring synthetic lethality and therapy sensitization.
These cells are ideally suited for tumorigenesis research, RNA biology investigations, and drug target validation. Typical experiments include RT-qPCR and western blotting to confirm knockout and downstream effects, RNA immunoprecipitation and RNA stability assays to examine mRNA regulation, and functional assays such as MTT/CCK-8 proliferation, Transwell migration/invasion, and apoptosis quantification. The polyclonal population also serves as a physiologically relevant system for Wnt/??-catenin signaling studies and RNA?Cprotein interaction analyses, with applications in xenograft tumor models and mechanistic dissection of metastatic pathways. For additional technical details and ordering information, please contact Ascent Research.
