The IGF2BP3 Knockout MCF-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population generated from the MCF-7 human breast adenocarcinoma cell line. This product features targeted disruption of the IGF2BP3 gene, encoding an RNA-binding protein that post-transcriptionally regulates oncogenic mRNAs. The polyclonal format provides a heterogeneous knockout pool, avoiding clonal bias and enabling robust loss-of-function studies in a luminal A breast cancer background.
MCF-7 cells are an estrogen receptor (ER)-positive, progesterone receptor (PR)-positive, HER2-negative breast cancer cell line derived from a metastatic pleural effusion of adenocarcinoma. They exhibit estrogen-dependent growth, recapitulating luminal A subtype features, making them a widely used model for hormone-responsive breast cancer research.
IGF2BP3 (Insulin-like Growth Factor 2 mRNA Binding Protein 3) is an oncofetal RNA-binding protein that enhances stability and translation of target mRNAs via 3??UTR binding. It is transcriptionally activated by MYC and the ??-catenin/TCF complex downstream of Wnt ligands (e.g., Wnt1, Wnt3a) and Frizzled receptors, and repressed by let?7 microRNAs. IGF2BP3 stabilizes transcripts such as MYC, CD44, and IGF2 mRNAs, interacting with HuR (ELAVL1) and RISC components. This regulation feeds into PI3K/AKT/mTOR and RAS/RAF/MEK/ERK signaling, driving proliferation and survival. The mRNA surveillance pathway intersects as IGF2BP3 protects mRNAs from degradation.
Knocking out IGF2BP3 in MCF-7 cells disrupts oncogenic mRNA stabilization, reducing levels of targets like MYC and CD44. In this ER?positive, luminal A context, knockout is expected to attenuate proliferation, migration, and invasion, while potentially sensitizing cells to endocrine therapy. The model enables dissection of post?transcriptional control crosstalk with hormone receptors and downstream MAPK/PI3K pathways.
This knockout pool is suited for Western blotting to confirm loss of IGF2BP3 and target proteins, RT?qPCR for transcript stability, RNA immunoprecipitation to assess protein?RNA interactions, and transwell migration/invasion and proliferation assays for functional readouts. RNA?seq and polysome profiling enable comprehensive transcriptomic and translatomic analyses. Typical applications include post?transcriptional gene regulation, cancer biology, metastasis mechanisms, and drug resistance research. For additional details or technical support, please contact Ascent Research.
