IKBIP Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with targeted disruption of the IKBIP gene. This loss-of-function model preserves the polyclonal nature of the edited pool, avoiding clonal selection artifacts. The use of CRISPR/Cas9 introduces genetic perturbations at the IKBIP locus, generating a heterogeneous knockout population suitable for functional screening and pathway analysis.
The host cell line HAP1 is a near-haploid human fibroblast-like cell line derived from KBM-7 chronic myeloid leukemia cells. Adapted for stable haploidy and adherent growth, HAP1 cells are widely employed in genetic screens due to their haploid genome, which simplifies gene disruption and enables clear phenotypic analyses. Their adherent morphology and rapid proliferation make them compatible with various cell-based assays.
IKBIP encodes a protein that directly interacts with IKBKB (IKK-??), a central kinase in the NF-??B signaling cascade. By inhibiting IKK-?? activity, IKBIP reduces the phosphorylation and degradation of NFKBIA (I??B??), thereby attenuating NF-??B transcriptional activation mediated by RELA. IKBIP is transcriptionally regulated by TP53 in response to DNA damage and promotes apoptosis through activation of caspases such as CASP3 and CASP9. Thus, IKBIP acts as a pro-apoptotic factor linking p53-mediated stress signaling to the cell death machinery.
In the HAP1 near-haploid background, disruption of IKBIP leads to loss of protein function, enabling clear phenotypic readouts for dissecting its role in NF-??B regulation and p53-dependent apoptosis. The haploid genome ensures that gene disruption results in functional consequences without allelic redundancy. This model allows robust interrogation of IKBIP??s impact on signaling crosstalk and apoptotic responses, with the fibroblast-like character supporting high-throughput imaging and flow cytometry.
This IKBIP knockout product supports diverse applications including NF-??B luciferase reporter assays, Western blotting for IKK-??, phospho-RELA, and IKBIP, and co-immunoprecipitation to probe protein interactions. Apoptosis can be quantified using Annexin V/PI staining, caspase activity measurements, and flow cytometry. The model is valuable for cancer biology studies and drug sensitivity testing with IKK inhibitors. For additional technical information or to discuss customized solutions, please contact Ascent Research.
