The IKBKE Knockout HEK293T Polyclonal Cells consist of a heterogenous population of HEK293T cells with CRISPR/Cas9-mediated disruption of the IKBKE gene, providing a loss-of-function model for studying IKBKE-dependent signaling. This polyclonal knockout product avoids clonal selection, offering a representative mixture of edited alleles suitable for functional genomics, pathway interrogation, and drug screening applications.
HEK293T is a human embryonic kidney cell line immortalized with adenovirus 5 DNA and expressing SV40 large T antigen, known for exceptional transfectability and recombinant protein expression. Its epithelial origin and permissiveness to genetic manipulation make it a standard platform for viral packaging, gene editing, and signal transduction research, supporting robust experimental reproducibility.
IKBKE encodes a serine/threonine kinase that functions as a non-canonical I??B kinase, activated by viral double-stranded RNA, LPS, and TLR3/TLR4 or RIG-I/MDA5 agonists. It directly phosphorylates IRF3 and IRF7 to drive type I interferon production and promotes NF-??B activation via p65/RELA. Additionally, IKBKE phosphorylates AKT and STAT1, contributing to cell survival and oncogenesis. The kinase operates in complexes with TBK1, adaptors TANK and TRAF3, and interacts with MAVS and DDX3, linking upstream pathogen recognition to downstream gene expression.
In the HEK293T background, IKBKE knockout impairs phosphorylation of IRF3, IRF7, STAT1, and AKT, diminishing interferon responses and altering PI3K/AKT and JAK-STAT signaling. This polyclonal model captures population-level effects on antiviral innate immunity and oncogenic pathways, enabling studies of pathway cross-talk and compensatory adaptation without the constraints of clonal variation.
Key applications include luciferase reporter assays for NF-??B and IFN-??, western blotting for phospho-IRF3, phospho-STAT1, and phospho-AKT, RT-qPCR for IFN-??, ISG15, and CXCL10, co-immunoprecipitation of IKBKE complexes, and RNA-seq transcriptomic profiling. The model supports cell proliferation, apoptosis, and xenograft studies for cancer biology, as well as antiviral drug target validation. For further information on custom knockout services, contact Ascent Research.
