The IL15RA knockout A-549 polyclonal cells are a mixed population of A-549 human lung adenocarcinoma cells with CRISPR/Cas9-mediated disruption of the IL15RA gene. This polyclonal knockout cell population preserves the parental genetic background while introducing a loss-of-function mutation of IL15RA, enabling reliable studies of IL-15/IL-15RA signaling in a relevant epithelial tumor model. The product provides a bulk knockout model free from clonal selection artifacts, suitable for diverse in vitro experimental designs.
A-549 is a human lung adenocarcinoma cell line established from a 58-year-old male, characterized by epithelial morphology, hypotriploid karyotype, and wild-type p53 expression. Widely used as a model of type II alveolar epithelial cells, it is a key tool in respiratory disease and cancer research, including drug metabolism, viral infection, and lung adenocarcinoma biology. This well-characterized background provides a consistent platform for investigating the role of IL15RA in tumor cell behavior.
IL15RA encodes the alpha subunit of the IL-15 receptor, which binds IL-15 and presents it to IL-2RB/IL-2RG, triggering JAK1/JAK3-mediated phosphorylation of STAT5 and STAT3 and activation of PI3K/AKT and RAS/MAPK pathways. The receptor complex engages adaptors TRAF6 and SHC, and downstream targets include BCL-2, MYC, cyclin D1, and SOCS1. IL15RA transcription is regulated by TNF-alpha, interferon-gamma, NF-??B, STAT5, and TGF-beta. In epithelial and immune cells, IL15RA modulates survival, proliferation, and cytokine output, influencing tumor?Cimmune crosstalk.
In A-549 lung adenocarcinoma cells, IL15RA knockout attenuates JAK/STAT, PI3K/AKT, and MAPK signaling downstream of IL-15, impairing cell survival, proliferation, and migration. Altered cytokine secretion, such as reduced IL-10, may reshape the tumor microenvironment and immune interactions. This model is valuable for dissecting the contribution of the IL-15/IL-15RA axis to lung cancer progression, immune evasion, and therapeutic resistance.
Research applications include lung cancer immune microenvironment analysis, cytokine signaling studies, and drug target validation for the IL-15/IL-15RA pathway. The cells support Western blotting for IL15RA and phospho-STAT5, RT-qPCR, flow cytometry, and functional assays for proliferation, apoptosis, migration, and invasion. Co-culture with immune cells and cytokine ELISA profiling further enable investigation of tumor?Cimmune dynamics. For technical inquiries, contact Ascent Research.
