The IL27 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population generated from the A2780 human epithelial ovarian cancer cell line, designed for loss-of-function studies of the IL27 gene. IL27 encodes interleukin-27, a cytokine that orchestrates immune regulation through a heterodimeric receptor composed of IL27RA and gp130 (IL6ST). Using CRISPR/Cas9-mediated gene disruption, these polyclonal cells provide a heterogeneous pool of edited cells, avoiding clonal selection artifacts while enabling robust interrogation of IL27-dependent signaling and tumor-immune crosstalk.
The parental A2780 cell line, originating from an untreated ovarian endometrioid adenocarcinoma, serves as an established model of ovarian carcinoma. These adherent epithelial cells retain key molecular features of ovarian cancer, including alterations in DNA repair and apoptotic pathways, and are widely employed to investigate tumor cell biology, drug responsiveness, and immune interactions within the ovarian tumor microenvironment.
IL27 functions by engaging its heterodimeric receptor to activate JAK-STAT signaling, predominantly through JAK1, JAK2, and TYK2 kinases that phosphorylate STAT1 and STAT3. Activated STAT1 and STAT3 transcriptionally regulate downstream targets including TBX21, IL10, SOCS1, and SOCS3. IL27 signaling is induced by upstream regulators such as LPS, IFN-??, CD40L, and transcription factors NF-??B, IRF1, and IRF8. It partners with EBI3 to form the functional cytokine, and its receptor complex shares the gp130 signal transducer with other IL-6 family cytokines. This pathway promotes Th1 differentiation and IFN-?? production while opposing Th17 responses and stimulating anti-inflammatory IL-10 secretion.
In the context of epithelial ovarian cancer, IL27 influences the immunological landscape by modulating the balance between pro-inflammatory and suppressive signals. A2780 cells express components of the IL27 signaling machinery, and knockout of IL27 in this line enables dissection of autocrine and paracrine cytokine effects on tumor cell behavior and immune cell recruitment. The model is particularly relevant for exploring how tumor-intrinsic cytokine production shapes the ovarian tumor microenvironment and for studying links to autoimmune conditions such as rheumatoid arthritis and inflammatory bowel disease, where IL27 plays regulatory roles.
This knockout product supports a broad range of experimental applications, including phospho-STAT1/STAT3 western blotting, RT-qPCR for TBX21 and IL10 transcript levels, flow cytometric analysis of surface receptors, and ELISA-based cytokine profiling. Co-culture systems with immune effectors can be designed to assess T-cell polarization and tumor cell killing, complemented by RNA-seq transcriptomic profiling to map global gene expression changes upon IL27 loss. These tools facilitate detailed investigation of JAK-STAT signaling dynamics, Th1/Th17 differentiation, and cytokine network interactions in ovarian cancer research. For additional product specifications and technical support, please contact Ascent Research.
