The IL27 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9?edited cell population generated from the Ca Ski human cervical carcinoma cell line, featuring targeted disruption of the IL27 gene. Supplied as a polyclonal pool, this model enables loss?of?function interrogation without clonal selection, facilitating robust studies of IL?27?dependent processes. The knockout eliminates the p28 subunit required for assembly of the functional IL?27 cytokine, thereby abrogating downstream signaling initiated by the IL?27 heterodimer.
The Ca Ski cell line is an epithelial, HPV?16?positive cervical carcinoma model originally derived from an epidermoid carcinoma metastasis. Extensively used in HPV oncogenesis research, Ca Ski cells retain integrated HPV?16 genomes and express viral oncoproteins E6 and E7, providing a clinically relevant context for investigating cytokine networks that intersect with viral transformation and immune escape.
IL27 encodes the IL?27 alpha subunit (p28), which partners with EBI3 to form the IL?27 heterodimeric cytokine. Secreted IL?27 binds a receptor complex of IL?27RA (WSX?1/TCCR) and gp130, triggering JAK1/JAK2?mediated phosphorylation of STAT1 and STAT3. Upstream activators include TLR agonists, IFN???, TNF???, and IL?1??, while downstream targets encompass SOCS1, SOCS3, T?bet, and IL?10. Through this pathway, IL?27 exerts context?dependent control over T?cell differentiation, inflammation, and immune regulation.
In the Ca Ski background, IL?27 signaling contributes to the modulation of the tumor microenvironment, potentially influencing immune surveillance and tumor progression. Disruption of IL27 permits dissection of how HPV?16 oncoproteins coordinate with IL?27?mediated STAT1/STAT3 activation to regulate checkpoint molecules, inflammatory mediators, and cellular functions such as migration and apoptosis. This knockout system thus offers a powerful model to explore the interplay between viral pathogenesis and IL?27 biology.
Applications include cytokine/chemokine profiling, phospho?STAT1/STAT3 flow cytometry, RT?qPCR analysis of IL?27 target genes, immune cell co?cultures, and migration/invasion assays. The polyclonal pool is also suitable for RNA?seq transcriptomic profiling and compound screening targeting the IL?27 pathway. For further technical information, please contact Ascent Research.
