The INO80C Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for loss-of-function studies of the INO80C gene in a HEK293T background. This pooled product contains a heterogeneous assortment of edited alleles, ensuring effective target gene disruption across the population and eliminating the need for single-cell cloning, thereby facilitating rapid deployment in chromatin biology and genome stability research.
The HEK293T host cell line is a human embryonic kidney epithelial line that constitutively expresses the SV40 large T antigen, contributing to its high transfection efficiency. It is widely used for recombinant protein production, lentivirus packaging, and transient gene expression, and its adherent morphology and robust growth support scalable experimental workflows including CRISPR-based gene editing and cell-based assays.
INO80C is a non-catalytic subunit of the INO80 ATP-dependent chromatin remodeling complex, which modulates nucleosome positioning to facilitate DNA repair, replication, and transcription. The INO80 complex includes interacting factors such as ACTR5, ACTR8, RUVBL1, RUVBL2, INO80B, and INO80E. Upstream, INO80C function is regulated by ATM and ATR kinases in response to DNA damage, and by E2F transcription factors. Downstream, it influences ??H2AX chromatin domain formation, RAD51 foci assembly, and chromatin accessibility at target genes, thereby impacting homologous recombination and gene expression programs.
In HEK293T cells, INO80C disruption impairs chromatin remodeling, leading to defective DNA damage responses and altered transcriptional regulation. This polyclonal knockout model is valuable for studying the links between nucleosome dynamics and genomic instability, a feature of cancer and developmental disorders. Phenotypic assays for DNA repair, cell cycle progression, and gene expression changes in these cells can illuminate chromatin-related disease mechanisms.
These cells support diverse applications, including functional dissection of INO80C in chromatin remodeling, mechanistic studies of DNA double-strand break repair, and drug sensitivity screening. Researchers can employ western blotting for INO80 complex subunits, ChIP-qPCR for histone modifications, immunofluorescence for ??H2AX and RAD51 foci, RNA-seq, cell cycle flow cytometry, comet assays, colony formation, and homologous recombination reporter assays. For technical details, contact Ascent Research.
