The IQGAP1 Knockout HAP1 Polyclonal Cells are a polyclonal knockout cell population generated by CRISPR/Cas9-mediated disruption of the IQGAP1 gene in the HAP1 cell line. This product provides a near-haploid cellular model lacking functional IQGAP1, suitable for studying its role in cytoskeletal dynamics and cell adhesion signaling. The polyclonal format avoids the limitations of single-cell cloning and preserves population heterogeneity, enabling robust loss-of-function experiments.
HAP1 is a human near-haploid cell line derived from the chronic myeloid leukemia KBM-7 line. It maintains an adherent growth pattern and a male karyotype. Its near-haploid genome simplifies gene editing and reduces genetic redundancy, making it an ideal chassis for knockout studies. Because HAP1 cells retain key signaling pathways of the myeloid lineage, they serve as a relevant platform to investigate the molecular mechanisms underlying leukemia and other cancers.
IQGAP1 is a scaffold protein that integrates signals from cell adhesion receptors and growth factor receptors to coordinate actin cytoskeleton remodeling and cell-cell adhesion. It directly interacts with calmodulin, ??-catenin, E-cadherin, N-cadherin, vinculin, Rac1, and Cdc42, linking cadherin-mediated adhesion to actin dynamics. Upstream activators include EGF, HGF, integrins, and Ca2+/calmodulin, while downstream targets are ERK1/2, JNK, and NF-??B. Through its association with Cdc42 and Rac1, IQGAP1 modulates MAPK/ERK and Wnt/??-catenin pathways, governing cell migration, proliferation, and differentiation.
In HAP1 cells, which originate from a leukemic background, disruption of IQGAP1 enables researchers to dissect its contributions to oncogenic signaling, particularly in the context of cell migration and invasion. The near-haploid nature ensures efficient knockout and simplifies downstream analyses, such as CRISPR screens and pathway reconstitution. This model is valuable for investigating how loss of IQGAP1 affects actin cytoskeletal organization and cadherin-based adhesion, critical in cancer metastasis and hematopoietic cell trafficking.
The IQGAP1 Knockout HAP1 Polyclonal Cells are suitable for western blotting, immunofluorescence, transwell migration, and wound healing assays to assess protein expression, cytoskeletal architecture, and cell motility. They also enable co-immunoprecipitation to examine protein complexes and GTPase activity assays to monitor Rac1/Cdc42 signaling. This model supports cancer biology, cardiovascular disease, and neurological disorder studies. For further details or ordering, contact Ascent Research.
