The ITGA3 Knockout HAP1 Polyclonal Cells represent a heterogeneous population of HAP1 human cells in which CRISPR/Cas9-mediated gene disruption has been used to ablate functional expression of the ITGA3 gene. This polyclonal knockout product provides a robust loss-of-function model for investigating integrin alpha-3 biology without the need for single-cell cloning, enabling rapid deployment in functional assays and genetic screens.
HAP1 is a near-haploid cell line derived from the KBM-7 chronic myeloid leukemia background, possessing a single copy of most chromosomes except for chromosome 8. This near-haploid state simplifies genetic manipulation and reduces the risk of compensatory paralog expression, making HAP1 an ideal host for systematic knockout studies. Its adherent growth and stable karyotype further support reproducible cell-based assays.
ITGA3 encodes the ??3 subunit of integrin ??3??1, a major receptor for laminin-332, collagen IV, and fibronectin. Ligand engagement triggers focal adhesion kinase (FAK) and Src activation, initiating downstream PI3K-Akt and MAPK/ERK signaling cascades that coordinate cell adhesion, migration, proliferation, and survival. The ??3??1 heterodimer functionally interacts with tetraspanin CD151 and uPAR (PLAUR) to regulate pericellular proteolysis and signal integration. Upstream, TGF-??, talin-1, and kindlin-2 modulate the activation state of the integrin, while downstream effectors include MMP9 and the transcription factor ZEB1, key drivers of epithelial-mesenchymal transition (EMT).
Knockout of ITGA3 in HAP1 cells removes ??3??1-mediated extracellular matrix sensing, yielding a genetically clean system to dissect integrin-dependent phenotypes. Because HAP1 cells express a limited integrin repertoire, ITGA3 disruption profoundly impairs adhesion to laminin and collagen substrates, alters focal adhesion dynamics, and suppresses migration. This model is thus highly relevant for studying the molecular basis of diseases linked to ITGA3 dysfunction, including interstitial lung disease, nephrotic syndrome, epidermolysis bullosa, and metastatic cancer.
These polyclonal knockout cells are ideally suited for a variety of research applications, such as high-throughput functional genomics screens, cell adhesion assays on laminin-coated surfaces, transwell migration assays, and drug sensitivity profiling. Users can validate ITGA3 knockout by Western blotting, RT-qPCR, or flow cytometry for surface integrin expression. Downstream signaling analyses may involve phospho-FAK detection, Src kinase assays, or RNA-seq transcriptional profiling. By providing a ready-to-use polyclonal knockout population, this product streamlines experimental workflows. For further technical information or purchasing support, please contact Ascent Research.
