ITGB1 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population derived from the Ca Ski cervical carcinoma cell line, featuring targeted disruption of the ITGB1 gene. The polyclonal knockout model comprises a heterogeneous pool of edited cells, providing a robust system for studying loss-of-function effects without clonal selection bias. This product is intended for researchers investigating integrin beta-1 function in adhesion, signaling, and cancer biology.
The Ca Ski line is an HPV-16-positive cervical squamous cell carcinoma originally isolated from a small intestine metastasis. These epithelial cells stably express HPV oncoproteins E6 and E7, which inactivate tumor suppressors p53 and Rb, making them a widely used model for cervical cancer progression and viral oncogenesis. Their metastatic origin renders them particularly suitable for studies on tumor dissemination and invasion.
ITGB1 encodes integrin beta-1, a subunit that heterodimerizes with various alpha integrins to form receptors for ECM ligands such as fibronectin, laminin, and collagen. Ligand binding triggers FAK and Src kinase activation, propagating signals through PI3K-Akt and MAPK/ERK pathways. Upstream regulators include TGF-??, EGF, and transcription factors AP-1 and NF-??B; key downstream effectors encompass FAK, Src, Akt, ERK1/2, RhoA, and Rac1. The cytoplasmic adaptors talin and kindlin are essential for integrin activation and linkage to the actin cytoskeleton.
In Ca Ski cells, ITGB1 contributes to HPV-16-driven malignant properties, such as enhanced adhesion, migration, and chemoresistance, likely through FAK and Akt signaling. Disrupting ITGB1 in this background allows dissection of integrin-specific contributions to cervical cancer cell behavior. This knockout model is valuable for revealing how ECM-integrin interactions intersect with viral oncoprotein activity to promote metastasis and drug resistance.
Typical applications include cell adhesion and Transwell migration/invasion assays, immunofluorescence visualization of focal adhesions, and Western blot analysis of downstream signals like phosphorylated FAK and ERK. The polyclonal population is also suited for drug sensitivity studies and RNA-seq profiling to uncover ITGB1-dependent transcriptional networks. For additional information, please contact Ascent Research.
