The ITGB3 Knockout A2780 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population in which the ITGB3 gene has been disrupted to eliminate integrin ??3 expression. This polyclonal format provides a heterogeneous pool of cells carrying varied loss-of-function alleles, enabling robust functional studies without clonal selection bias.
Derived from the A2780 human ovarian epithelial carcinoma cell line, these cells were established from a primary untreated adenocarcinoma and retain wild-type TP53 and BRCA1 status. The A2780 line is widely used as a model of high-grade serous ovarian carcinoma and maintains a chemosensitive phenotype, making it particularly valuable for studies of drug response and resistance mechanisms.
ITGB3 encodes integrin ??3, a subunit that heterodimerizes with ??v or ??IIb to form the ??v??3 and ??IIb??3 receptors. These integrins recognize RGD-containing ligands such as vitronectin and fibronectin, and are activated by talin-1 and kindlin-3. Upon ligand binding, ITGB3 mediates outside-in signaling through FAK (PTK2) autophosphorylation and SRC activation, triggering downstream cascades including PI3K?CAKT1 and MAPK1/3 (ERK1/2). The receptor also interacts with VEGFR2 and PDGFR, and signals via the FAK?CSrc?Cp130Cas complex to regulate RHOA, RAC1, and transcription factors such as NF-??B and AP-1, controlling cell adhesion, migration, and survival.
In ovarian cancer, ITGB3 overexpression is correlated with enhanced peritoneal dissemination, anoikis resistance, and poor prognosis. The A2780 background, with its intact p53 and BRCA1 pathways, allows dissection of ITGB3-dependent processes in a chemosensitive context, providing a platform to investigate how integrin ??3 signaling contributes to tumor aggressiveness and drug susceptibility.
This polyclonal knockout model is suited for a range of functional assays, including cell adhesion to vitronectin or fibronectin, wound-healing migration, Transwell invasion, and anoikis assays. Researchers can employ these cells in Western blot analyses for ITGB3, FAK, and phosphorylated FAK, or in phospho-kinase arrays to map signaling alterations. They are also applicable in flow cytometry for surface ??v??3, in chemosensitivity testing with cisplatin or paclitaxel, and in co-culture models of angiogenesis and platelet?Ctumor cell interactions. For further information or to inquire about custom services, please contact Ascent Research.
