ITM2B Knockout A-549 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from A-549 human lung adenocarcinoma cells. This product features targeted disruption of the ITM2B (BRI2) gene, generating a loss-of-function model suitable for investigating ITM2B-dependent processes. The polyclonal format provides a heterogeneous pool of edited genes for bulk assays without clonal bias.
A-549 cells, established from lung adenocarcinoma tissue, represent a widely used type II alveolar epithelial model. These cells retain malignant properties, including rapid proliferation, migration, and invasion, while maintaining key epithelial characteristics. Their well-studied genome and relevance to non-small cell lung cancer make them an optimal platform for genetic perturbation studies, particularly for interrogating pathways involved in tumorigenesis and metastasis.
ITM2B encodes a type II transmembrane protein cleaved by furin and ADAM10, releasing a secreted fragment that interacts with APP and regulates the gamma-secretase complex composed of PSEN1, PSEN2, nicastrin, APH-1, and PEN-2. Through this interaction, ITM2B modulates amyloid-beta production and Notch receptor processing, influencing NICD-mediated transcription. Additionally, ITM2B is implicated in cell adhesion and migration, connecting it to both neurodegeneration and cancer phenotypes.
In the A-549 background, ITM2B knockout enables dissection of gamma-secretase activity and Notch signaling within a cancer-relevant context. While ITM2B is traditionally associated with Alzheimer??s and familial dementias, its impact on cell migration and adhesion suggests roles in metastatic progression. This model thus bridges neurobiology and oncology, allowing researchers to examine how a neuroprotective factor affects lung adenocarcinoma behavior.
Typical applications include gamma-secretase activity assays using APP or Notch substrates, amyloid-beta ELISA, co-immunoprecipitation with gamma-secretase components, and cell migration/invasion analyses. Researchers can also perform western blotting and RT-qPCR for knockout validation, as well as Notch reporter assays. For custom inquiries or additional product details, please contact Ascent Research.
