JADE2 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population in the near-haploid HAP1 human cell line. This product provides a loss-of-function model for JADE2 (PHF16), a key transcriptional coactivator. The polyclonal knockout pool is generated by CRISPR/Cas9-mediated gene disruption, enabling studies of JADE2 deficiency without clonal selection bias, suitable for investigating chromatin biology and cell proliferation roles.
HAP1 cells are a fibroblastoid, male, near-haploid human cell line derived from KBM-7 chronic myeloid leukemia cells. Their haploid karyotype simplifies knockout generation, making them a preferred platform for genetic perturbation studies. The near-haploid nature enables efficient gene disruption in polyclonal populations. HAP1 cells maintain functional cancer-related pathways, including Wnt signaling and chromatin regulation, and support diverse functional assays, serving as a versatile host for studying HBO1 acetyltransferase complex components.
JADE2 scaffolds the HBO1 acetyltransferase complex, comprising the catalytic subunit KAT7 (HBO1) and cofactors ING4, ING5, MEAF6, and BRPF1. This complex acetylates histone H3 at lysine 14 (H3K14ac) and histone H4, regulating transcriptional programs for cell proliferation and differentiation. JADE2 is activated by Wnt signaling via TCF/LEF transcription factors and growth factor pathways. Downstream, it promotes expression of cell cycle genes CCND1 and MYC, and influences developmental gene expression by modulating the chromatin landscape. JADE2 interacts with transcription factors for context-specific gene regulation.
In the HAP1 model, JADE2 disruption permits dissection of its role in the HBO1 complex and its effects on histone acetylation dynamics. Given the role of H3 and H4 acetylation in transcription, this knockout system enables analysis of how JADE2-dependent modifications influence cell cycle progression and proliferation. The haploid background aids in detecting phenotypic changes without allele masking, providing a robust functional genomics system. Additionally, with HAP1’s leukemia origin, this model supports studies of JADE2 in hematopoietic malignancies and therapeutic target validation.
Typical applications include ChIP-qPCR for histone acetylation marks like H3K14ac, western blotting for H4 acetylation, and RT-qPCR for downstream targets CCND1 and MYC. Proliferation assays and colony formation evaluate JADE2 loss effects on cell growth. The cells are suitable for haploid genetic screens and drug sensitivity profiling to find synthetic lethal interactions, as well as CRISPR-based functional genomics or RNA-seq to map transcriptional consequences. For further information, contact Ascent Research.
