The JADE3 Knockout HEK293T Polyclonal Cells provide a polyclonal knockout cell population generated through CRISPR/Cas9-mediated gene disruption of the JADE3 locus in the widely utilized HEK293T human embryonic kidney cell line. This product delivers a heterogeneous loss-of-function model in which target-gene disruption is achieved across the cell population, enabling functional interrogation of JADE3 without clonal isolation. The polyclonal format is particularly suited for pooled screening approaches and studies requiring representation of diverse editing outcomes.
HEK293T cells are a well-established adherent epithelial cell line derived from human embryonic kidney and are distinguished by stable expression of the SV40 large T antigen, which confers markedly enhanced transfection efficiency and supports high-level protein expression. These attributes make HEK293T a preferred host for a broad range of applications, including viral vector production, transient and stable transfections, and signaling pathway analyses. The robust growth characteristics and well-characterized transcriptomic profile of HEK293T further underpin its utility in mechanistic studies.
JADE3 encodes a crucial scaffold protein within the HBO1 histone acetyltransferase (HAT) complex, where it interacts directly with HBO1 (KAT7), BRPF proteins, and ING5 to orchestrate the acetylation of histone H3 and H4. This histone modification relaxes chromatin architecture and activates transcription of downstream target genes such as CCND1 and MYC, which are critical for cell cycle progression and proliferation. JADE3 function is itself subject to regulation by upstream developmental signals and cell cycle cues, positioning it as a key node linking extracellular stimuli to chromatin remodeling and gene expression programs.
The ablation of JADE3 in HEK293T cells creates a powerful system for dissecting its contributions to histone acetylation dynamics and transcriptional control within a kidney epithelial context. Given the roles of JADE3 and the HBO1 complex in cancer and neurodevelopmental disorders, this knockout model provides a versatile platform for investigating epigenetic dysregulation. Researchers can employ these cells to explore how JADE3 loss impacts chromatin state, gene expression networks, and cellular phenotypes relevant to oncogenic transformation and developmental anomalies.
This polyclonal knockout product supports a wide array of downstream applications, including quantitative chromatin immunoprecipitation (ChIP-qPCR) to assess histone H3/H4 acetylation levels, RT-qPCR and RNA-seq for transcriptomic profiling, and Western blotting for protein-level validation. Cell proliferation assays and co-immunoprecipitation experiments enable functional interrogation of HBO1 complex integrity and growth phenotypes. The model is also amenable to epigenetic drug screening and cancer signaling studies. For additional details and technical support, please contact Ascent Research.
