This product is a CRISPR/Cas9-edited polyclonal JAG2 knockout cell population derived from the Ca Ski human cervical carcinoma cell line. The JAG2 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population designed to disrupt expression of the JAG2 gene in the Ca Ski host cell line. This knockout model provides a powerful tool for investigating Jagged2-dependent signaling in a well-characterized cervical cancer background.
Ca Ski cells are a human cervical epidermoid carcinoma line that is positive for human papillomavirus type 16 (HPV-16). These epithelial cells are widely used as a model for cervical cancer and HPV-mediated oncogenesis, offering a relevant context for studying molecular mechanisms of tumorigenesis, viral integration, and host-virus interactions.
JAG2 encodes Jagged2, a single-pass transmembrane ligand for Notch receptors (NOTCH1?C4). Upon cell-cell contact, Jagged2 activates Notch signaling, triggering ADAM protease-mediated cleavage followed by ??-secretase-dependent release of the Notch intracellular domain (NICD). NICD translocates to the nucleus, where it forms a complex with the CSL transcription factor and coactivators to regulate target gene expression. Downstream transcriptional targets include the Hes and Hey families, Myc, and p21. Notch signaling is modulated by glycosyltransferases such as Lunatic Fringe, which modify receptor sensitivity to ligands. Upstream, JAG2 expression is regulated by transcription factors including E2F and NF-??B, and by cytokines such as TGF-??. The Jagged2-Notch pathway is central to cell fate decisions, epithelial-to-mesenchymal transition, and stem cell maintenance.
In Ca Ski cells, which harbor integrated HPV-16 genomes and constitutively express viral oncogenes E6 and E7, JAG2 knockout provides a precise genetic background to dissect the interplay between Notch signaling and HPV-driven transformation. The polyclonal nature preserves heterogeneity while eliminating Jagged2-dependent signaling, enabling studies of how Notch pathway alterations influence cervical cancer cell proliferation, apoptosis, migration, and invasion. This model is especially valuable for examining crosstalk between Notch and other oncogenic pathways in a cervical carcinoma setting.
Researchers can employ these cells in a variety of functional assays, including western blotting for JAG2 and NICD, RT-qPCR analysis of Notch target genes, reporter assays using Notch-responsive luciferase constructs, immunofluorescence for Jagged2 localization, and flow cytometric assessment of Notch receptor activation. Additional applications include apoptosis assays, migration and invasion studies, and drug screening to identify Notch pathway modulators. The JAG2 knockout Ca Ski cells are suitable for investigating cervical cancer biology, Notch-mediated cell differentiation, and developmental signaling. For further information, please contact Ascent Research.
