The JAK2 Knockout HCT 116 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population engineered for loss-of-function studies of the JAK2 gene in the HCT 116 colorectal carcinoma line. The polyclonal format, generated by CRISPR/Cas9-mediated gene disruption, comprises a heterogeneous mix of edited cells, minimizing clonal bias and enabling robust assessment of JAK2-dependent phenotypes. This product is designed for advanced research requiring disruption of JAK-STAT signaling in a well-characterized colorectal cancer model.
HCT 116 is a human colorectal carcinoma epithelial cell line derived from a male patient, widely employed as a model for colorectal cancer biology and drug discovery. The cells retain key oncogenic features, including deregulated proliferative signaling and apoptotic resistance, making them a relevant host for investigating oncogenic kinase networks. Their adherent growth and well-annotated genome facilitate molecular analyses following gene perturbation.
JAK2 encodes a non-receptor tyrosine kinase that transduces signals from cytokine receptors such as EPOR, MPL, and IL-6R. Upon activation, JAK2 phosphorylates STAT3 and STAT5, which dimerize and regulate transcription of genes like MYC and CCND1; it additionally activates PI3K/AKT and MAPK/ERK cascades. Negative regulators include SOCS1, SOCS3, PTPN6, and PIAS proteins. In HCT 116 cells, JAK2 mediates cytokine-driven proliferation and survival, and its knockout abrogates downstream STAT and AKT/ERK activation.
Aberrant JAK2 activity contributes to colorectal tumor growth and inflammatory signaling. The JAK2 knockout polyclonal cells enable dissection of JAK2??s role in cytokine-induced oncogenesis, independent of clonal artifacts. This model supports screening of JAK2 inhibitors and evaluation of pathway-specific dependencies in a solid tumor context, aiding the development of targeted therapies for colorectal cancer.
Research applications include western blotting and phospho-flow cytometry for JAK2/STAT pathway analysis, RT-qPCR and RNA-seq for gene expression profiling, and functional assays such as MTT proliferation, Annexin V apoptosis, colony formation, and migration/invasion tests. Co-immunoprecipitation and luciferase reporter assays further delineate JAK2 interaction networks and transcriptional responses. For additional technical support, please contact Ascent Research.
