The JPH1 Knockout A-549 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with disruption of the JPH1 gene, providing a loss-of-function model for junctophilin-1. This polyclonal pool avoids single-cell cloning, preserving genetic diversity while ensuring consistent knockout. The cells are supplied as a ready-to-use culture, amenable to expansion and cryopreservation.
The host A-549 cell line, derived from a 58-year-old Caucasian male with lung adenocarcinoma, is an established model for lung cancer and alveolar epithelial biology. Exhibiting type II pneumocyte features and carrying KRAS and TP53 mutations, A-549 cells are widely used to study oncogenic signaling, drug responses, and calcium dynamics, providing a robust platform for gene-edited derivatives.
JPH1 encodes junctophilin-1, a structural tether between the plasma membrane and the endoplasmic/sarcoplasmic reticulum essential for calcium handling. JPH1 interacts with DHPR/CACNA1S, RYR1, caveolin-3, and TRPC3, organizing calcium release units. Its expression is regulated by MyoD, myogenin, and MEF2, linking it to muscle differentiation. Downstream, JPH1 facilitates coupling of voltage-gated calcium channels to RYR1-mediated release and modulates SERCA activity. JPH1 disruption uncouples these interactions, impairing efficient calcium signaling.
In A-549 cells, JPH1 knockout serves as a tool to dissect membrane contact site roles in non-excitable cancer cells. Although JPH1 is mainly studied in excitable tissues, its epithelial expression suggests functions in calcium-dependent processes like proliferation, migration, and apoptosis. Loss of JPH1 may perturb calcium microdomains, affecting calmodulin and CaMKII and altering cellular responses. This model helps assess JPH1??s contribution to tumorigenic phenotypes or drug sensitivity in lung adenocarcinoma.
Applications include calcium imaging (Fluo-4, Fura-2), proliferation (BrdU/EdU), wound-healing and Transwell migration, and apoptosis (Annexin V) assays. RNA-seq, Western blotting, and immunofluorescence enable pathway characterization. These cells support small-molecule screening and tumor-stroma co-culture studies. For further details, contact Ascent Research.
