This product provides a CRISPR/Cas9-edited polyclonal knockout cell population targeting the JPT1 gene in HEK293T cells. The polyclonal format ensures a heterogeneous loss-of-function model that reflects the variability of gene disruption, making it suitable for pooled functional studies. This knockout model has not been clonally isolated, offering a population-level assessment of JPT1 deficiency.
The host cell line HEK293T is a widely used human embryonic kidney epithelial derivative that stably expresses the SV40 large T antigen, enabling high-level episomal replication of plasmids containing the SV40 origin of replication. This feature makes HEK293T cells an ideal platform for transfection, protein overexpression, and lentiviral production. Their robust growth and ease of culture further support large-scale experiments in cell cycle and microtubule biology.
JPT1 encodes a microtubule-associated protein that directly binds tubulin and interacts with other MAPs, including MAP1S and MAPRE1, to promote microtubule stabilization and proper mitotic spindle organization. It functions downstream of as-yet-unidentified upstream regulators to influence microtubule dynamics, thereby regulating cell division. Disruption of JPT1 may impair spindle assembly, leading to mitotic defects and altered proliferation, consistent with its role in cancer-related pathways.
In the HEK293T context, JPT1 knockout provides a tractable system to dissect microtubule-dependent processes. The ease of transfection allows complementation with wild-type or mutant JPT1 constructs, while the rapid cell cycle facilitates live-cell imaging of mitotic events. Loss of JPT1 may sensitize cells to microtubule-targeting agents, offering a platform for drug target validation and exploring synthetic lethality in cancer models.
Researchers can employ this polyclonal knockout population in a wide array of applications, including Western blot analysis of JPT1 and phospho-histone H3, immunofluorescence microscopy to assess microtubule morphology, flow cytometry for cell cycle profiling, and live-cell imaging to track mitotic progression. Transcriptional profiling via RT-qPCR for mitotic genes and clonogenic assays for long-term proliferation can further define the functional consequences of JPT1 loss. For additional details or to discuss custom applications, please contact Ascent Research.
