KCNK3 Knockout SKOV3 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Ovary
Disease:
Ovarian serous cystadenocarcinoma

Datasheet

The KCNK3 Knockout SK-OV-3 Polyclonal Cells provide a loss-of-function model for TASK-1 (K2P3.1) two-pore domain potassium channels in a human ovarian adenocarcinoma epithelial cell line. This polyclonal knockout population enables investigation of KCNK3 signaling, including its regulation by Gq-coupled receptors and protein kinase C, downstream effects on membrane depolarization and apoptosis, and interactions with 14-3-3 proteins. Applications include electrophysiology, drug screening for modulators, and studies of hypoxia and pH responses in ovarian cancer.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

SKOV3

Sex of Donor

Female

Age

64 years

Derived From Site

Ascites

Gene Name

KCNK3

Gene Identifier

NCBI Gene ID 3777

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

McCoy's 5A

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

This product comprises a CRISPR/Cas9-edited polyclonal knockout cell population in which the KCNK3 gene has been disrupted to eliminate functional TASK-1 (K2P3.1) potassium channels.

The polyclonal format provides a heterogeneous pool of edited cells, enabling robust loss-of-function studies without requiring clonal isolation.

This model is designed for researchers investigating the roles of background potassium leak currents in ovarian cancer cell biology, with emphasis on membrane potential regulation, calcium signaling, and adaptive responses to the tumor microenvironment.