The KDM2B Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HEK293T human embryonic kidney cell line, engineered for targeted disruption of the KDM2B gene. This polyclonal pool allows loss-of-function studies without clonal selection bias, enabling investigation of KDM2B??s roles in epigenetic regulation, signaling, and oncogenesis within a broadly used, transfection-competent background. The product supports reproducible functional assays by averaging heterogeneous edit outcomes representative of population-level effects.
HEK293T cells originate from HEK293 cells stably transformed with adenovirus 5 DNA and expressing the SV40 large T antigen, which enables episomal replication of plasmids carrying the SV40 origin. This feature, combined with their embryonic kidney epithelial phenotype, yields exceptionally high transfection efficiency and robust protein production, making them a standard platform for recombinant expression, lentiviral packaging, and functional genomics. The line’s genetic tractability and rapid growth further benefit high-throughput screening applications.
KDM2B encodes a histone lysine demethylase that selectively removes dimethylation from H3K36 (H3K36me2), modulating chromatin structure and transcription. Crucially, it serves as a core component of the non-canonical polycomb repressive complex 1 (ncPRC1), interacting with RING1B, BMI1, PCGF1, RYBP, and CBX7 to recognize unmethylated CpG islands and catalyze H2AK119 monoubiquitination, driving gene silencing. Its activity is regulated by MYC, E2F1, p53, TGF-?? signaling, miR-101, and the miR-30 family. Downstream targets include CDKN2A, CDKN1A, CCND1, CDH1, and HOXA gene clusters. Through these interactions, KDM2B exerts control over cell cycle progression, apoptosis, and stem cell maintenance, and its dysregulation is implicated in colorectal, breast, and other cancers.
In the HEK293T background, KDM2B knockout enables mechanistic dissection of its dual catalytic and scaffolding functions. The cell line??s high transfectability allows efficient re-expression of KDM2B variants for structure-function studies, while the polyclonal nature captures heterogeneous epigenetic responses that better reflect native tissue environments. This model is particularly valuable for investigating crosstalk between the PRC1 system, Wnt/??-catenin pathway components including ??-catenin, and TGF-??/SMAD2/3 signaling, all of which converge on cell fate decisions and oncogenic processes.
Research applications include chromatin immunoprecipitation?CqPCR (ChIP-qPCR) to map H3K36me2 and H3K27me3 changes, combined with RNA-seq and RT-qPCR for transcriptome profiling. Functional assays such as flow cytometry for cell cycle distribution, apoptosis detection, and colony formation quantify KDM2B??s impact on proliferation and survival. Co-immunoprecipitation validates interactions with ncPRC1 members like RING1B and BMI1. The knockout also supports drug target validation and functional genomics screens in colorectal cancer, acute myeloid leukemia, glioblastoma, and neurodevelopmental disorders. For further information, contact Ascent Research.
